线粒体功能的检测方法

The detection methods of mitochondrial function

线粒体为细胞内的能量工厂,对细胞的增殖、分化、存活以及稳态的维持起着重要的调节作用。线粒体功能障碍与机体生长、发育异常、认知发生障碍以及多种器官病变密切相关。线粒体形态、结构和功能的检测对于了解线粒体的稳态以及功能状态有着重要意义,线粒体的功能状态与线粒体膜电位、线粒体膜通道、线粒体Ca2+浓度、ATP生成、呼吸链复合体活性、活性氧生成以及DNA突变密切相关。本文就线粒体形态、结构和功能的检测方法及其研究进展进行了综述。

Mitochondria are intracellular energy factories that play important roles in regulating cell proliferation, differentiation, survival, and homeostasis. Mitochondrial dysfunction is closely related to the abnormal of growth, differentiation, cognitive impairment, and multiple organ diseases. The detection of mitochondrial morphology, structure and function is important for understanding the mitochondrial homeostasis and functional status. Mitochondrial functional status is related to mitochondrial membrane potential, mitochondrial membrane channels, mitochondrial Ca2+ concentration, ATP production, respiratory chain complex activity, reactive oxygen species production and DNA mutation. This review summerized the progress of detection methods on mitochondrial morphology, structure and function.