60Co-γ射线辐照灭菌对沉香化气胶囊中6个挥发性成分的影响

Effect of 60Co irradiation on 6 volatile components in Chenxiang Huaqi capsules

目的:建立气相色谱法同时测定沉香化气胶囊中木香烃内酯、去氢木香烃内酯、百秋李醇、广藿香酮、α-香附酮和乙酸龙脑酯6个挥发性成分的含量,考察不同剂量60Co-γ射线辐照前后挥发性成分的变化。方法:采用Agilent HP-1301(30 m×0.32 mm×0.25μm)色谱柱,柱温采用程序升温,进样口温度280℃,检测器温度300℃,分流比35:1,检测器为氢火焰离子化检测器,载气为氮气,进样量1μL。分别选择辐照剂量2、5、8 kGy对沉香化气胶囊进行辐照,比较辐照前后沉香化气胶囊中6个挥发性成分含量的变化,并进行成组t-检验考察其显著情况。结果:木香烃内酯、去氢木香烃内酯、百秋李醇、广藿香酮、α-香附酮和乙酸龙脑酯分别在0.06153~3.0769、0.06001~3.0005、0.04327~2.1635、0.04173~2.0865、0.05098~2.5490及0.05251~2.6259μg范围内有线性关系良好,平均加样回收率分别为100.9%、101.1%、99.9%、98.9%、101.4%和101.3%,RSD分别为0.73%、0.56%、0.86%、1.0%、0.61%和1.1%。经过2、5、8 kGy辐照后,沉香化气胶囊所含的挥发性成分木香烃内酯、去氢木香烃内酯、百秋李醇、广藿香酮、α-香附酮和乙酸龙脑酯均有变化,经配对t-检验,超过5 kGy后,木香烃内酯及去氢木香烃内酯辐照前后含量变化显著(P<0.05)。结论:所建立的沉香化气胶囊挥发性成分测定方法回收率高,重复性好,灵敏度高,可作为沉香化气胶囊质控方法。在辐照剂量不超过5 kGy时,各挥发性成分变化不显著,可为沉香化气胶囊辐照灭菌手段提供参考。

Objective:To establish a GC method for simultaneous determination of 6 volatile components in Chenxiang Huaqi capsules including costunolide,dehydrocostuslactone,patchoulialcohol,pogostone,α-cyperone and borneol acetate,so as to study the effect of 60 Co-γray irradiation on the volatile components of Chenxiang Huaqi capsules.Methods:The capillary column Agilent HP-1301(30 m×0.32 mm×0.25μm)was adopted and the column temperature was controlled by programming.The injector temperature was 280℃and the detector temperature was 300℃.The split ratio was 35∶1 and the injection volume was 1μL.The detector was a hydrogen flame ionization detector.Chenxiang Huaqi capsules were irradiated at doses of 2,5 and 8 kGy.The contents of the 6 volatile components before and after irradiation were compared,and a t-test study was used to examine the significant condition.Results:The linear range of costunolide,dehydrocostuslactone,patchoulialcohol,pogostone,α-cyperone and borneol acetate were 0.06153-3.0769,0.06001-3.0005,0.04327-2.1635,0.04173-2.0865,0.05098-2.5490 and 0.05251-2.6259μg,respectively.And the average recovery were 100.9%,101.1%,99.9%,98.9%,101.4%and 101.3%with RSDs of 0.73%,0.56%,0.86%,1.0%,0.61%and 1.1%,respectively.After irradiation at doses of 2,5 and 8 kGy,contents of costunolide,dehydrocostuslactone,patchoulialcohol,pogostone,α-cyperone and borneol acetateare changed.By group t-test,contents of costunolide and dehydrocostuslactone changed significantly(P<0.05)after irradiation at doses above 5 kGy.Conclusion:The established method has high recovery rate,good repeatability and high sensitivity,which can be used for quality control of Chenxiang Huaqi capsules.When the radiation dose is not more than 5 kGy,the changes of each volatile components are not significant.The finding provides a reference for the sterilization of Chenxiang Huaqi capsules.