利舒康胶囊对模拟高原缺氧大鼠肺组织损伤的保护作用

Protective Effect of Lishukang Capsule on Lung Tissue Damage of Rats Exposed to Simulated High-altitude Hypoxia

目的探讨利舒康胶囊对模拟高原缺氧大鼠肺组织损伤的保护作用。方法选取72只雄性Wistar大鼠随机分为常氧对照组、缺氧模型组、红景天胶囊组、利舒康胶囊低、中、高剂量组,每组12只。红景天胶囊组给予红景天胶囊灌胃,利舒康胶囊低、中、高剂量组分别给予利舒康胶囊280、420、560 mg/kg灌胃,给药7 d。第7天给药50 min后,将大鼠放入大型低压氧舱模拟海拔8000 m高原缺氧12 h,常氧对照组不缺氧。观察6组大鼠肺组织损伤程度,同时检测大鼠肺组织中缺氧应激相关生化指标。结果利舒康高剂量组可明显缓解高原缺氧大鼠肺组织损伤。与常氧对照组比较,缺氧模型组大鼠肺组织谷胱甘肽过氧化物酶(GSH-PX)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、Na^+-K^+-ATP酶和Ca^2+-Mg^2+-ATP酶显著下降,丙二醛(MDA)、过氧化氢(H2O2)、一氧化氮合酶(NOS)、乳酸(LD)、乳酸脱氢酶(LDH)显著升高(P<0.05,P<0.01);与缺氧模型组比较,利舒康胶囊高剂量组GSH-PX、GSH、CAT、Na^+-K^+-ATP酶、Ca^2+-Mg^2+-ATP酶显著升高,MDA、H2O2、NOS、LD、LDH显著降低(P<0.05,P<0.01)。结论利舒康对模拟高原缺氧大鼠肺损伤有很好的保护作用,其机制可能与清除机体过多的自由基,提高抗氧化能力,改善能量代谢有关。

Objective To investigate the protective effect of Lishukang Capsule on lung tissue damage of rats exposed in simulated high-altitude hypoxia.Methods Seventy-two male Wistar rats were randomly divided into normal control group, hypoxia model group, Rhodiola group, low, medium and high-dose Lishukang groups, with 12 rats in each group. Rhodiola group was given Rhodiola capsule by gavage, while low, medium and high-dose Lishukang groups were given Lishukang capsule 280, 420 and 560 mg/kg by gavage for 7 d. At 50 min after administration on the 7 th day, the rats were placed in a large hypobaric chamber to simulate hypoxia for 12 h at an altitude of 8000 m, while normal control group was not treated with hypoxia. The lung tissue damage of rats was observed in six groups, and the biochemical indexes related to hypoxia stress in lung tissue of rats were detected. Results The high-dose Lishukang group had significantly alleviated lung tissue damage in rats exposed to high altitude hypoxia. Compared with the normal control group, GSH-PX, GSH, CAT, Na^+-K^+-ATP and Ca^2+-Mg^2+-ATP of the lung tissue of the hypoxia model group were decreased significantly, whereas MDA, H2O2, NOS, LD, LDH were increased significantly(P<0.05, P<0.01). Compared with the hypoxia model group, GSH-PX, GSH, CAT, Na^+-K^+-ATP and Ca^2+-Mg^2+-ATP in the high-dose Lishukang group were increased significantly, while MDA, H2O2, NOS, LD, LDH were decreased significantly(P<0.05, P<0.01). Conclusion Lishukang capsule has a good protective effect on lung damage induced by simulated high-altitude hypoxia. The mechanism may be related to the removal of excessive free radicals, improvement of antioxidant capacity and improvement of energy metabolism.