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蚕沙化学成分体外降解研究 被引量:11

Study on degradation of silkworm excrement chemical components in vitro
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摘要 该文旨在研究蚕沙被排出家蚕体外后的化学成分降解,并确定主要的降解成分及其变化关系。该研究基于UPLC-Q-TOF-MS技术,在系统解析蚕沙所含主要化学成分的基础上,采用主成分分析(PCA)和偏最小二乘法判别分析(OPLS-DA)方法对市售蚕沙和新鲜蚕沙进行差异分析,通过OPLS-DA的S-plot图筛选出对差异贡献较大的化学成分并鉴定,同时通过Masslynx对差异成分的相对峰面积进行提取,比较不同时间段收集的新鲜蚕沙内差异成分的相对含量。结果表明,新鲜蚕沙和市售蚕沙化合物组成具有明显差异,差异成分主要是黄酮苷类、Diels-Alder型化合物,而且2类化合物在蚕沙的存储过程中有降解现象。该研究首次采用质谱法对蚕沙化学成分进行系统的鉴定分析,并发现蚕沙在存放过程中部分化学成分发生降解。 The purpose of this article is to study the degradation of chemical compositions after the silkworm excrement being expelled from the silkworm,and to determine its main metabolic compositions and their changing relationships.This research is based on UPLC-Q-TOF-MS technology.Based on the systematic analysis of the main chemical compositions contained in silkworm excrement,the principal compositions analysis(PCA)and partial least squares discriminant analysis(OPLS-DA)on commercial silkworm excrement and fresh silkworm excrement were analyzed for differences.The S-plot chart of OPLS-DA was used to select and identify the chemical compositions that contributed significantly to the difference.At the same time,the relative peak areas of the different compositions were extracted by Masslynx to obtain the relative content of different compositions in fresh silkworm excrement.The results showed that there was a significant difference in the chemical compositions between fresh silkworm excrement and commercial silkworm excrement.The difference compositions were mainly flavonoid glycosides and Diels-Alder type composition,and two types of compounds are degradated during the storage of silkworm sand.In this study,the chemical compositions of fresh silkworm excrement were systematically identified and analyzed for the first time by mass spectrometry,and it was found that some chemical compositions of silkworm excrement were degradated with time during storage.
作者 张悦 邓爱平 翁倩倩 林王敏 詹志来 黄璐琦 ZHANG Yue;DENG Ai-ping;WENG Qian-qian;LIN Wang-min;ZHAN Zhi-lai;HUANG Lu-qi(Henan University of Chinese Medicine,Zhengzhou 450046,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Academician Workstation of Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Zhejiang Chinese Medical University,Hangzhou 310053,China)
出处 《中国中药杂志》 CAS CSCD 北大核心 2020年第9期2130-2137,共8页 China Journal of Chinese Materia Medica
基金 中国中医科学院基本科研业务费(ZZ13-YQ-091) 中央本级重大增减支项目(2060302) 国家重点研发计划项目(2017YFC1700805)。
关键词 蚕沙 化学成分 主成分分析 差异分析 成分降解 silkworm excrement chemical composition principal component analysis difference analysis composition degradation
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