不同中药乙醇提取物对酪氨酸酶抑制活性的比较

Comparative study on the tyrosinase inhibitory effect of some ethanol extracts of traditional Chinese medicine

目的 对几味中药醇提物的美白活性进行研究,为开发中药美白产品提供实验依据.方法 高良姜、白及、白芷、苏木、白术、紫草、艾叶、马齿苋、诃子9味中药采用70%乙醇提取方法 ,以维生素C和熊果苷为阳性对照,采用体外酪氨酸酶抑制法实验,比较不同药物的活性差异.结果 中药诃子酪氨酸酶抑制率为(51.43±2.52)%,高于熊果苷的(46.34±6.32)%.中药艾叶和马齿苋也有一定的抑制酪氨酸酶作用,抑制率分别为(22.88±7.99)%和(13.07±11.98)%.而其他几味中药几乎没有抑制酪氨酸酶作用.结论 中药诃子、艾叶醇提物对酪氨酸酶有一定的抑制活性,可作为美白中药进一步研究.

Objective To study the whitening activity of ethanol extracts of several traditional Chinese medicine,and to provide experimental basis for the development of whitening products of traditional Chinese medicine.Methods Nine traditional Chinese medicine including lesser galangal rhizome,common bletilla rubber,dahurian angelica root,sappan wood,largehead atractylodes rhizome,Radix Arnebiae,argy wormwood leaf,purslane herb,and medicine terminalia fruit were extracted with 70%ethanol.Vitamin C and arbutin were used as positive control drugs.In vitro tyrosinase inhibition test was employed to compare the bioactivity of different drugs.Results The inhibition rate of traditional Chinese medicine medicine terminalia fruit was(51.43±2.52)%,was higher than(46.34±6.32)%of arbutin.Artemisiae Argyi,Herba Portulacae also have good tyrosinase inhibity,the inhibition rates were(22.88±7.99)%and(13.07±11.98)%,respectively.However,the other Chinese medicine hardly inhibited tyrosinase activity.Conclusion The ethanol extracts of medicine terminalia fruit and argy wormwood leaf have certain inhibitory activities on tyrosinase,which can be further studied as whitening tradiational Chinese medicine.