Mina53与CBX8在肝细胞肝癌中的表达及其与临床病理特征和预后的关系

Expression of Mina53 and CBX8 in Hepatocellular Carcinoma and Their Correlation with Clinicopathological Features and Prognosis

目的探讨Mina53与CBX8在肝细胞肝癌中的表达,并分析两者与肝细胞肝癌患者临床病理特征及预后的关系。方法收集121例肝细胞肝癌患者术后切除标本制作组织芯片,采用免疫组织化学PV6000法检测Mina53与CBX8在肝细胞肝癌及相应癌旁相对正常组织中的表达,应用SPSS23.0软件对数据进行统计分析。结果Mina53、CBX8在肝细胞肝癌中的表达水平高于癌旁相对正常组织(均P<0.001)。Mina53表达与肿瘤大小、被膜侵犯、脉管侵犯、病理分级、TNM分期有关(均P<0.05);CBX8表达与乙肝表面抗原(HBsAg)、肿瘤大小、被膜侵犯、TNM分期有关(均P<0.05)。肝细胞肝癌中Mina53与CBX8表达呈正相关(r=0.574,P<0.01)。Mina53与CBX8均高表达患者的OS和DFS较Mina53或CBX8高表达者和两者均低表达者更短(均P<0.001)。多因素分析显示,Mina53、CBX8的表达水平是肝细胞肝癌患者术后OS和DFS的独立预后影响因素(P<0 . 0 5 ) 。结论 Mina53及CBX8在肝细胞肝癌组织中高表达, 可能与肝细胞肝癌的发生发展和浸润转移有关,Mina53和CBX8高表达提示患者预后不良,联合检测Mina53和CBX8有助于肝细胞肝癌的预后判断。

Objective To investigate the expressions of Mina53 and CBX8 in hepatocellular carcinoma(HCC),and to analyze their correlation with the clinicopathological features and prognosis of HCC patients.Methods A total of 121 samples of HCC obtained by surgical resection were collected for tissue microarray construction.The expressions of Mina53 and CBX8 were detected by immunohistochemical PV6000 method in HCC and corresponding adjacent non-tumor tissues.Statistical analysis was performed by using SPSS23.0 software.Results The expressions of Mina53 and CBX8 were both significantly higher in HCC than those in the adjacent non-tumor tissues(both P<0.001).Expression of Mina53 was correlated with tumor size,capsular invasion,vascular invasion,tumor differentiation and TNM stage(all P<0.05).Expression of CBX8 was closely correlated with HBsAg,tumor size,capsular invasion and TNM stage(all P<0.05).The expressions of Mina53 and CBX8 were positively correlated(r=0.574,P<0.01).Patients with high expressions of both Mina53 and CBX8 had shorter overall survival and disease-free survival than those with high expression of Mina53 or CBX8 or both low expressions of Mina53 and CBX8(P<0.05).Multivariate analyses showed that Mina53 and CBX8 expressions were independent influence factors for overall survival and disease-free survival of HCC patients(P<0.05).Conclusion The high expression of Mina53 and CBX8 may be correlated with the pathogenesis,invasion and metastasis of HCC.Patients with high expressions of both Mina53 and CBX8 have a poor prognosis,which indicates that the combined detection of Mina53 and CBX8 is conducive for predicting the prognosis of HCC patients.