首发未用药精神分裂症患者肠道菌群构成及其与血清IL-6、IL-10、TNF-α水平的关系

Intestinal flora of patients untreated first-episode schizophrenia and its relationship with levels of IL-6,IL-10 and TNF-αin serum

目的探讨首发未用药精神分裂症患者肠道菌群构成及其与血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)水平的关系。方法抽取2016年1月至2018年1月太原市精神病医院收治的首发未用药精神分裂症患者268例作为观察组,选取同期体检的健康者110例作为对照组。两组研究对象均采用酶联免疫吸附法检测IL-6、IL-10、TNF-α水平,比较乳酸杆菌、双歧杆菌与拟杆菌、肠球菌构成比,采用Pearson相关性分析首发未用药精神分裂症患者肠道菌群构成与IL-6、IL-10、TNF-α水平的关系。结果观察组患者IL-6、IL-10、TNF-α水平高于对照组健康体检者(P<0.05);观察组肠道菌群中乳酸杆菌、双歧杆菌菌落数少于对照组(P<0.05),拟杆菌、肠球菌菌落数多于对照组(P<0.05);观察组患者乳酸杆菌/拟杆菌、乳酸杆菌/肠球菌、双歧杆菌/拟杆菌、双歧杆菌/肠球菌低于对照组(P<0.05);首发未用药精神分裂症患者乳酸杆菌/拟杆菌、乳酸杆菌/肠球菌、双歧杆菌/拟杆菌、双歧杆菌/肠球菌与IL-6、IL-10、TNF-α水平呈负相关(r<0,P<0.05)。结论首发未用药精神分裂症患者血清IL-6、IL-10、TNF-α水平升高,肠道菌群失衡,且肠道菌群失衡与血清炎性因子水平变化关系密切,两者在精神分裂症发生过程中均有重要作用。

Objective To investigate the relationship between intestinal flora and serum levels of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor α(TNF-α) in patients with untereated first-episode schizophrenia.Methods A total of 268 patients with untreated first-episode schizophrenia in Taiyuan Psychiatric Hospital from January 2016 to January 2018 were selected as the observation group, and 110 healthy people in the same period were selected as the control group. The levels of IL-6, IL-10 and TNF-α were detected by enzyme-linked immunosorbent assay (ELISA) in the two groups. The composition of Lactobacillus, Bifidobacterium, Bacteroides and Enterococcus were compared. The relationship between the composition of intestinal flora and the levels of IL-6, IL-10 and TNF-α was analyzed by Pearson correlation.Results The levels of IL-6, IL-10 and TNF-α in the observation group were higher than those in the control group (P<0.05);the number of Lactobacilli and Bifidobacteria in the intestinal flora of the observation group was lower than that of the control group (P<0.05). The numbers of Bacteroides and Enterococcus were higher than those of the control group (P<0.05). Lactobacillus/Bacteroides, Lactobacillus/Enterococcus, Bifidobacterium/Bacteroides and Bifidobacterium/Enterococcus in the observation group were lower than those in the control group (P<0.05). Lactobacilli/Bacteroides, Lactobacilli/Enterococcus, Bifidobacteria/Bacteroides, Bifidobacteria/Enterococcus were negatively correlated with the levels of IL-6, IL-10, TNF-α (r<0, P<0.05).Conclusions The levels of IL-6, IL-10 and TNF-α in serum of patients with first-episode schizophrenia were increased, and the imbalance of intestinal flora was closely related to the change of serum inflammatory factors, both of which played important roles in the development of schizophrenia.