罗氏Modular P800全自动生化分析仪使用低密度脂蛋白胆固醇试剂对载脂蛋白A1检测比色杯携带污染的干扰效应

Interference Effect of LDL-C Reagent by Roche Modular P800 Analyzer on the Colorimetric Cup Carried Pollution Detected by ApoA1

目的探讨罗氏Modular P800全自动生化分析仪使用低密度脂蛋白胆固醇(LDL-C)试剂对载脂蛋白A1(ApoA1)检测比色杯携带污染的干扰效应。方法使用罗氏公司和积水公司的LDL-C试剂盒在罗氏Modular P800全自动生化分析仪上分别检测不同浓度的LDL-C试剂对罗氏ApoA1检测的影响,并进行分析。结果罗氏LDL-C试剂在Modular P800上对罗氏ApoA1的检测有明显干扰,且干扰程度随着LDL-C浓度的增加而增大;积水LDL-C试剂在Modular P800上对罗氏ApoA1的检测无干扰;酸性洗液和去离子水清洗均能够消除罗氏LDL-C试剂对罗氏ApoA1检测比色杯携带污染的干扰。结论全自动生化分析仪存在比色杯的携带污染,影响结果的准确性,实验室可通过特殊清洗程序排除携带污染的影响,且应主动发现并排除携带污染,保证检测结果的准确性。

Objective To investigate the interference effect of LDL-C of Roche Modular P800 biochemical analyzer on ApoA1 colorimetric cup carrying pollution,and to find an effective solution.Methods Roche Modular P800 automatic biochemical analyzer was used to detect the effects of LDL-C in different concentrations on the detection of Roche ApoA1 with LDL-C reagent from Roche and Sekisui company,and then statistical analysis was performed.Results Roche LDL-C reagent significantly interfered with the detection of ApoA1 on Roche Modular P800,and the interference increased with the increase of LDL-C concentration;Sekisui LDL-C reagent did not interfere with the detection of ApoA1 on two automatic biochemical analyzers.Both acid cleaning solution and deionized water cleaning could eliminate the interference of Roche LDL-C reagents on ApoA1 detection of colorific cup contamination.Conclusions There is contamination of the colorimetric cup in the biochemical analyzer,which affects the accuracy of the results.The laboratory can eliminate the influence of contamination by special cleaning procedures,and should take the initiative to detect and eliminate contamination to ensure the accuracy of the results.