重组人粒细胞集落刺激因子对急性肺损伤幼鼠炎症因子表达的影响

Effect of recombinant human granulocyte colony-stimulating factor on the expression of inflammatory factors in young rats with acute lung injury

目的探讨重组人粒细胞集落刺激因子(rhG-CSF)对急性肺损伤幼鼠炎症因子表达的影响。方法选取120只SD幼鼠,随机分为实验组和对照组,每组60只。采用经尾静脉注射精制大肠杆菌内毒素脂多糖5 mg/kg建立急性肺损伤模型。实验组采用皮下注射rhG-CSF 30μg/(kg·d)进行干预,对照组则注射同等剂量的生理盐水,比较两组肺组织及支气管肺泡灌洗液在干预后第5、10、14天转化生长因子β1(TGF-β1)、肿瘤坏死因子α(TNF-α)和白介素1β(IL-1β)的表达水平。结果在实验干预后第5、10、14天,实验组支气管肺泡灌洗液及肺组织的TGF-β1、TNF-α和IL-1β水平均低于对照组,差异有统计学意义(P<0.05)。结论rhG-CSF能够有效地减轻急性肺损伤幼鼠的机体炎性反应。

Objective To investigate the effect of recombinant human granulocyte colony-stimulating factor(rhG-CSF)on the expressions of inflammatory factors in young rats with acute lung injury.Methods One hundred and twenty young Sprague-Dawley(SD)rats were selected and randomly divided into experimental group and control group,with 60 cases in each group.A model of acute lung injury was established by injection of refined lipopolysaccharide of escherichia coli endotoxin 5 mg/kg via tail vein.The experimental group was treated with subcutaneous injection of rhG-CSF 30μg/(kg·d),and the control group was injected with the same volume of normal saline.The levels of transforming growth factor-β1(TGF-β1),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in lung tissues and bronchoalveolar lavage fluid were compared between the two groups on the 5th,10th,and 14th days after the intervention.Results On the 5th,10th and 14th days after the experimental intervention,the levels of TGF-β1,TNF-αand IL-1βin bronchoalveolar lavage fluid and lung tissues in the experimental group were significantly lower than those in the control group(P<0.05).Conclusion rhG-CSF can effectively reduce the body′s inflammatory response of young rats with acute lung injury.