抗坏血酸插层铁锆氧化物的制备及急性毒性评价

Preparation and Acute Toxicity Evaluation of Ascorbic Acid Intercalated Iron-zirconium Oxide

目的以松针提取液为绿色溶剂合成铁锆氧化物并对抗坏血酸进行负载和缓释,为提高其生物利用度提供科学依据。方法通过单一因素实验探讨该材料的制备条件对抗坏血酸负载率的影响,并利用体内实验和体外实验评价该材料的急性毒性抗坏血酸插层铁锆氧化物的缓释效果。结果当三氯化铁和氯氧化锆的摩尔比为8:1、加入0.5 g十六烷基三甲基溴化铵、反应温度为60℃和烘干时间为24 h时,制备的铁锆氧化物对抗坏血酸的最大负载率为97.82%。该材料对实验小鼠没有明显急性毒性,抗坏血酸插层铁锆氧化物在模拟胃液和肠液中最大释放时间均为450 min。结论该方法合成的铁锆氧化物适宜作为抗坏血酸的缓释载体。

Objective To co-load ascorbic acid with iron zirconium oxide,which was synthesized using pine needle extract as green solvent,to provide scientific basis for improving ascorbic acid bioavailability.Methods The influence of preparation condition of iron-zirconium oxide on ascorbic acid loading rate was studied by a single factor test,and acute toxicity of this material and the sustained-release property of ascorbic acid intercalated iron-zirconium oxide were evaluated by in vivo in vitro tests.Results When the molar ratio of ferric chloride and zirconium oxychloride was 8:1,0.5 g cetyltrimethylammonium bromide was added,the reaction temperatures and drying time were 60℃and 24 h,respectively,the maximum loading ratio of ascorbic acid on iron-zirconium oxide was achieved at 97.82%.The material had no obvious acute toxicity to experimental mice.The maximum release times of ascorbic acid intercalated iron zirconium oxide in both stimulated gastric juice and intestinal juice were 450 min.Conclusion The iron-zirconium oxide synthesized by this method was suitable as the sustained-release carrier for ascorbic acid.