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马齿苋/甘草/蒲公英及其复方改善2型糖尿病大鼠胰岛素抵抗的实验研究 被引量:8

Study of purslane/licorice/dandelion compound on insulin resistance in type 2 diabetic rats
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摘要 目的探讨马齿苋、甘草、蒲公英及其复方对2型糖尿病大鼠胰岛素抵抗的情况。方法小剂量链脲佐菌素联合高糖高脂饮食建立2型糖尿病大鼠模型,造模成功后随机分为模型组、马齿苋组、甘草组、蒲公英组、复方组及二甲双胍组并设正常组。干预4周后,测定各组大鼠FBG、TC、TG、HDL、LDL、FINS数值的变化并计算AI值、ISI值和IRI值。取胰腺组织制成切片,染色后观察胰岛细胞的形态学变化。结果 (1)各单方和复方干预组体重与模型组比较均具有显著性差异(P <0.01),但效果不及二甲双胍。(2) 2周时,各干预组大鼠FBG值与模型组比较差异具有统计学意义(P <0.05);4周时,各干预组大鼠FBG值均显著降低(P <0.01);干预2周以及干预4周复方组与二甲双胍组比较差异不具有统计学意义(P> 0.05)。(3)复方组和二甲双胍组糖负荷后0.5、1、2 h血糖值均显著降低,但2 h血糖比较有统计学差异(P <0.05)。(4)各干预组与模型组比较ISI值显著升高、IRI值显著降低(P <0.01),复方组IRI值与二甲双胍组比较差异无统计学意义(P> 0.05)。(5)复方组血清TC、TG、LDLC值显著降低(P <0.01),HDH-C值显著升高(P <0.01),同时发现二甲双胍组TC、TG、HDH-C和LDL-C水平与复方组相似(P> 0.05)。(6)显微镜下观察发现复方组有修复增生表现,细胞排列均匀,大小一致,与二甲双胍组相似。结论马齿苋、甘草、蒲公英复方可调节糖脂代谢紊乱、修复胰岛β细胞从而改善胰岛素抵抗。整体效果优于单方。 Objective To explore the effects of purslane,licorice,and dandelion compound on insulin resistance in type 2 diabetic rats. Methods Low-dose streptozotocin was combined with a high-sugar and high-fat diet to establish type 2 diabetic rats. After successful modeling,they were randomly divided into control( normal),model,purslane,licorice,dandelion,compound,and metformin groups. After 4 weeks of intervention,Fasting blood glucose( FBG),Serum total cholesterol( TC),Triglyceride( TG),High-density lipoprotein( HDL),Low-density lipoprotein( LDL),and Fasting insulin( FINS) were measured in each group of rats. The changes in the values were calculated and Atherosclerosis index( AI),Insulin sensitivity index( ISI),and Insulin resistance index( IRI) values were calculated. The pancreatic tissue was cut into sections and the morphological changes were observed after staining. Results( 1) The weight of each intervention group was significantly different from that of the model group( P< 0. 01),but the effect was not as good as metformin.( 2) At 2 weeks,the FBG value of each intervention group was significantly lower than that of the model group( P< 0. 05);at 4 weeks,the FBG value of each intervention group was significantly lower( P< 0. 01),and there was no statistical significance between the compound group and the metformin group before and after 2 weeks and 4 weeks of intervention( P > 0. 05).( 3) The 0. 5,1,and 2 h blood sugar values of the compound and metformin groups were significantly lower after the glucose load,but there was a significant difference in 2-h blood glucose( P< 0. 05).( 4) The ISI and IRI values of each intervention group were significantly higher and lower than those of the model group,respectively( P< 0. 01).( 5) The levels of TC,TG,HDL-C,and LDL-C in the metformin group were similar to those in the compound group( P> 0. 05).( 6) Microscopic observation revealed that the compound group underwent repair and proliferation,and the cells were arranged evenly and uniformly in size,similar to those in the metformin group. Conclusions Purslane,licorice,and dandelion compound can regulate glucose and lipid metabolism disorders,repair islet β cells,and thereby improve insulin resistance. The overall effect of combination treatment is better than that achieved with single treatment.
作者 李敏 杨晨钰 傅超慧 熊一功 凌云 LI Min;YANG Chenyu;FU Chaohui;XIONG Yigong;LING Yun(College of Medical Laboratory,Changsha Medical College,Changsha 410219,China)
出处 《中国实验动物学报》 CAS CSCD 北大核心 2020年第4期517-524,共8页 Acta Laboratorium Animalis Scientia Sinica
基金 湖南省大学生创新创业训练计划项目(湘教通[2019]219号-2410) 湖南省教育厅科研项目(17CO176) 湖南省卫生计生委基金科研课题(C20180131)。
关键词 马齿苋 甘草 蒲公英 复方 降血糖 胰岛素抵抗 胰岛Β细胞 purslane glycyrrhiza dandelion compound hypoglycemic insulin resistance isletβcell
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