摘要
Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).
目的寻找多基源黄精中共有的指标性成分并建立定性定量方法。方法以液相色谱-质谱(LC-MS)分析黄精中的共同主要成分,并在其指导下用柱层析色谱对其分离纯化,利用核磁共振(NMR)和LC-MS对其结构进行鉴定。参考2015版《中国药典》中薄层色谱鉴定方法,并在此基础上对其条件进行优化。结果成功从黄精浸膏中分离出共同主要成分并鉴定为亚油酸,建立的亚油酸高效液相色谱(HPLC)含量测定方法线性相关系数R2=0.9997,精密度、稳定性、重复性和加样回收率实验均符合要求。经条件优化后,黄精薄层色谱鉴定质量明显提高,并能鉴定黄精中共有主要成分亚油酸。结论不同品种黄精中主要成分亚油酸含量差异较大,姜型黄精中亚油酸含量明显高于鸡头黄精和大黄精。优化后的黄精薄层色谱方法简便可行,可用于黄精质量控制。
基金
We thank for the funding support from the National Standardization Construction in TCMs of China(No.ZYBZH-Y-HUN-23)
National Key Research and Development Projects of China(No.2018YFC1707903)
Key Research and Development Projects of Hunan Province(No.2018SK2119).
