反枝苋水提物对高脂饮食小鼠的降血脂作用

Effect of Water Extract of Amaranthus retroflexus on Blood Lipid on Mice Induced by High Fat Diet

目的:研究反枝苋水提物调节高脂血症小鼠血脂的功效。方法:通过高脂肪饮食(high fatdiet,HFD)构建高脂血症小鼠模型,随机分为空白对照组(Con)、高脂模型组(Mod)、洛伐他汀组(Lovastatin,10 mg/kg/d)、反枝苋低剂量组(AR-L,430 mg/kg/d)、反枝苋高剂量组(AR-H,740 mg/kg/d)。空白组给予普通饲料喂养,其余各组给予高脂饲料喂养,同时按相应剂量灌胃给药。干预8周后,检测血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein Cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)水平,肝脏HE染色观察组织病理学变化。结果:与空白组相比,模型组小鼠肝脏指数以及血清中TC、TG、LDL-C、ALT、AST、MDA水平极显著升高(P<0.01),HDL-C、SOD水平极显著降低(P<0.01);与模型组比较,反枝苋高剂量组小鼠的肝脏指数以及血清中TC、TG、ALT水平极显著降低(P<0.01),SOD、HDL-C水平极显著升高(P<0.01),LDL-C、MDA、AST水平显著降低(P<0.05);与模型比较,反枝苋低剂量组小鼠的肝脏指数以及血清中TC、TG、LDL-C水平显著降低(P<0.05),SOD、HDL-C水平极显著升高(P<0.01),ALT水平极显著降低(P<0.01),MDA、AST水平无显著性差异(P>0.05);HE染色结果显示,反枝苋水提物可明显减轻肝细胞内脂质沉积和肝脏脂肪变性程度。结论:反枝苋水提物能显著降低高脂血症模型小鼠的血脂水平,并能改善其肝功能。

Objective:To study the effect of water extract of Amaranthus retroflexus on hyperlipidemia mice.Methods:The mice model of hyperlipidemia was established by high fat diet(HFD).The mice were randomly divided into five groups:Control group(Con),model group(Mod),lovastatin group(Lovastatin,10 mg/kg/d),low dose of water extract of Amaranthus retroflexus group(AR-L,430 mg/kg/d)and high dose of water extract of Amaranthus retroflexus group(AR-H,740 mg/kg/d).The control group was fed with basic feed,the rest groups were fed with high-fat feed,and the drug was given by gavage according to the dosage.After eight weeks,the contents of serum total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),superoxide dismutase(SOD),malondialdehyde(MDA),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)levels in mice were determined.Mice liver histopathology was observed using hematoxylin and eosin(HE)staining.Results:Compared with the control group,the mice liver index extremely significantly increased(P<0.01)in the model group.The levels of mice serum TC,TG,LDL-C,ALT,AST and MDA significantly elevated and HDL-C,SOD extremely significantly reduced(P<0.01).Compared with the model group,the mice liver index extremely significantly increased(P<0.01)in the AR-H group.The levels of mice serum TC,TG and ALT extremely significantly reduced and SOD,HDL-C extremely significantly elevated(P<0.01),and the levels of mice serum MDA,AST significantly reduced(P<0.05).Compared with the model group,the mice liver index significantly increased(P<0.05)in the AR-L group.The levels of mice serum TC,TG and LDL-C significantly reduced(P<0.05),and the levels of mice serum SOD and HDL-C significantly elevated(P<0.01)and ALT extremely significantly reduced(P<0.01),and there were no significant difference in the levels of MDA and AST between the model group and AR-L group(P>0.05).The results of HE staining showed that the water extract of Amaranthus retroflexus could significantly reduce the lipid deposition and fatty degeneration of liver cells.Conclusion:The research shows that the water extract of Amaranthus retroflexus can significantly reduce the blood lipid level of hyperlipidemia model mice and improve their liver function.