利用响应面回归设计研制降解黄曲霉毒素和玉米赤霉烯酮的复合益生菌

Preparation of compound probiotics for degrading aflatoxin and zearalenone by response surface regression design

为了得到降解黄曲霉毒素B1(aflatoxin B1,AFB1)和玉米赤霉烯酮(zearalenone,ZEA)效果最佳的复合益生菌组合,选用对霉菌毒素降解效果好的枯草芽孢杆菌、干酪乳杆菌以及产朊假丝酵母,在1.0×10^5、1.0×10^6、1.0×10^7 CFU/ml三个水平上进行响应面回归设计。试验结果表明,枯草芽孢杆菌、干酪乳杆菌以及产朊假丝酵母组合为1×10^7、1×10^5、1×10^6 CFU/ml时,对ZEA降解率最高,达到36.42%(P<0.05);组合为1×10^6、1×10^7、1×10^7 CFU/ml时,对AFB1降解率最高,达到49.58%(P<0.05)。

In order to obtain the ideal combination of probiotics for degrading AFB1 and ZEA respectively,three species such asBacillus subtilis,Lactobacillus casei andCandida utilis with good mycotoxin-degrading ability were selected,and response surface regression design was used at 3 levels of 1.0×10^5,1.0×10^6 and 1.0×10^7 CFU/ml.The results showed that ZEA degradation rate was 36.42%(P<0.05)when the viable counts of Bacillus subtilis,Lactobacillus casei and Candida utilis were 1×10^7,1×10^5 and 1×10^6 CFU/ml;AFB1 degradation rate was 49.58%(P<0.05)at 1×10^6,1×10^7 and 1×10^7 CFU/ml.