摘要
目的探讨抑制卵巢癌细胞周期蛋白依赖性激酶(cyclindependent kinases,CDK6)基因后人卵巢癌细胞凋亡变化。方法将特异性CDK6-shRNA-608重组质粒和无关系列载体质粒转染人HO-8910卵巢癌细胞,并设立空白对照组;采用实时定量反转录PCR(real-time RT-PCR)方法检测转染后48 h各组HO-8910细胞CDK6 mRNA表达情况;流式细胞束检测各组HO-8910细胞凋亡的变化。结果特异性shRNA-608重组质粒转染HO-8910细胞48 h时CDK6 mRNA表达受到明显抑制,shRNA-608组细胞的凋亡率显著增加,与无关序列转染组和空白对照组相比,差异有显著性统计学意义(P<0.01)。结论抑制CDK6基因可促进卵巢癌HO-8910细胞的凋亡。
Objective To investigate the effect of CDK6 gene inhibition on the apoptosis of human ovarian cancer HO-8910 cells.Methods Specific CDK6-shRNA-608 recombinant plasmid and unrelated sequence shRNA were transfected in human HO-8910 cells,and the normal cells was set up as a blank control group.The expression of CDK6 mRNA in HO-8910 cells in different groups was detected by real-time RT-PCR at 48 hours post transfection.The apoptosis of HO-8910 cells in different groups were detected by FACS.Results Expression of CDK6 gene mRNA in HO-8910 cells was significantly inhibited by CDK6 shRNA-608 at 48 hours post transfection.The cell apoptosis increased significantly in shRNA-608 group,compared with the unrelated sequence group and the blank control group,the difference was significant(P<0.01).Conclusion CDK6 gene inhibition can promote apoptosis of HO-8910 cells in ovarian cancer.
作者
段丽
王莉莉
冯雪
王冠
邢焱玲
孙杰
颜莹
DUAN Li;WANG Lili;FENG Xue;WANG Guan;XING Yanling;SUN Jie;YAN Ying(Department of Medical Education,Heilongjiang Provincial Hospital,Harbin Heilongjiang 150036,China;Department of Reproductive Medicine,The First Affiliated Hospital of Harbin Medical University,Harbin Heilongjiang 150001,China;Department of Oncology,Harbin First Hospital,Harbin Heilongjiang 150001,China)
出处
《中国卫生标准管理》
2020年第21期130-132,共3页
China Health Standard Management
基金
黑龙江省卫生健康委科研课题(2018326)。
