协同纳微秒的全固态脉冲发生器研制

Development of All-solid-state Pulse Generator for Generating Synergistic Nanoseconds/Microseconds Pulses

高压窄脉冲与低压宽脉冲的协同方式可显著增强不可逆电穿孔(irreversible electroporation,IRE)的杀伤效果,扩大消融体积。为了深入研究协同脉冲增强不可逆电穿孔杀伤机制,设计了一种能灵活产生高压纳秒–低压微秒的协同脉冲序列的全固态脉冲发生器拓扑结构;基于现场可编程门阵列,搭建了满足控制时序要求的固态开关控制平台;最终研制了一套灵活产生协同脉冲的全固态脉冲发生器,并进行了样机的性能测试与实验验证。结果表明:该脉冲发生器能有效产生协同脉冲序列并增强细胞杀伤效果;该脉冲源可输出的纳秒脉冲参数为:电压幅值0~10 kV可调、脉宽100 ns^1μs可调、在100Ω负载下电流幅值可达100 A,微秒脉冲参数为:电压幅值0~3 kV可调、脉宽5~100μs可调、在100Ω负载下电流幅值可达30 A,且纳秒与微秒脉冲间隔时间任意可调。经过实验验证,全固态协同脉冲发生器的输出参数、样机性能满足协同脉冲诱导IRE相关生物实验需求,对于进一步研究协同脉冲的生物电学效应奠定了硬件基础。

The synergistic manner of high-pressure narrow pulse and low-pressure wide pulse can significantly enhance the killing effect of irreversible electroporation(IRE) and expand the ablation volume. In order to deeply study the killing mechanism of synergistic pulse-enhanced irreversible electroporation, we designed an all-solid-state pulse generator topology that can flexibly generate high-voltage nanosecond and low-voltage microsecond cooperative pulse sequence. Based on field programmable gate array, a solid-state switch control platform was built to meet the requirements of controlling the timing requirements;finally, a set of all solid-state pulse generators with flexible generation of coordinated pulses were developed, and the performance tests and experimental verification of the prototype were carried out. The results show that the pulse generator can effectively generate synergistic pulse sequences and enhance cell killing effect. The nanosecond pulse parameters that can be output by the pulse source are as follows: voltage amplitude 0~10 kV adjustable, pulse width 100 ns^1 μs adjustable, and current amplitude up to 100 A under 100 Ω load;meanwhile,the microsecond pulse parameters are as follows: voltage amplitude 0~3 kV adjustable, pulse width 5~100 μs adjustable, and current amplitude up to 30 A under 100 Ω load, and nanosecond and microsecond pulse interval can be adjusted arbitrarily. The experimental results show that the output parameters and prototype performance of the all-solid-state synergistic pulse generator meet the requirements of collaborative pulse-induced IRE-related biological experiments, which lays a hardware foundation for further study of the bioelectrical effects of synergistic pulses.