HPLC-MS/MS法分析水介质下F-53B的含量

Analysis of F-53B by HPLC-MS/MS

建立HPLC-MS/MS方法用于水介质中F-53B的含量检测。采用高效液相色谱-串联质谱法,试样用曝气生态水溶解,使用Agilent 1290-6470A LC/MS/MS测定,以20%水(0.1%甲酸)+80%乙腈为流动相,Eclipse plus C 18 RRHD柱,外标法定量。该分析方法的F-53B相关系数为0.9998,变异系数为0.64%,平均回收率分别101.4%。该方法操作简单,准确度、精密度及灵敏度均满足要求,可用于F-53B于水中含量检测。为F-53B在水环境中的检测,提供了一种可靠的方案。

A HPLC-MS/MS method to detect F-53B was established.The separation and quantitative analysis of F-53B the samples were conducted by HPLC-MS/MS and MSD detector with the mobile phase of HPLC was methanol and 0.1%formic acid-water solution(20:80,by vol)as mobile phase,and Eclipse plus C 18 RRHD column as fix phase.The results showed that the linear correlation coefficients for F-53B was 0.9998,the variation coefficients was 0.64%,the average recovery rates was 101.4%,respectively.The method was simple,the accuracy,precision and sensitivity were all satisfy the standard,which was suitable for analysis of F-53B.It provided a reliable scheme for the detection of F-53B in water environment.