高效液相色谱法同时测定奶昔粉中的安赛蜜和瑞鲍迪苷A

Determination of acesulfame potassium and rebaudioside A in milkshake powder by high performance liquid chromatography

目的建立高效液相色谱法(high performance liquid chromatography,HPLC)同时快速检测奶昔粉中安赛蜜和瑞鲍迪苷A含量的分析方法。方法样品用水溶解稀释,加入沉淀剂,离心过滤得样品供试液。采用C18色谱柱,以硫酸铵溶液:乙腈:甲醇=65:30:5(V:V:V)作为流动相,检测波长为210nm,流速0.6mL/min,进行检测,外标法定量。结果本方法在20min内完成2种目标化合物的分离分析。试样中安赛蜜浓度在0.0040~0.0403 mg/mL的范围内与峰面积的线性关系良好,相关系数r>0.999,在3水平不同浓度安赛蜜标准溶液添加下,安赛蜜的回收率为98.58%。试样中瑞鲍迪苷A浓度在0.0028~0.0283mg/mL的范围内与峰面积的线性关系良好,相关系数r>0.999。在3水平不同浓度瑞鲍迪苷A标准溶液添加下,瑞鲍迪苷A的回收率为99.04%。均符合标准GB/T27404-2008《实验室质量控制规范食品理化检测》中的要求,且目标峰分离效果更好,效率提升49.5%。结论该方法快速、准确、灵敏,适合同时测定奶昔粉中安赛蜜和瑞鲍迪苷A含量。

Objective To establish a method for simultaneous determination of acesulfame potassium and rebaudioside A in milkshake powder by high performance liquid chromatography.Methods The sample was diluted with water,after the precipitant was added,the sample was centrifuged and filtered to obtain the sample test solution.Ammonium sulfate solution:acetonitrile:methanol=65:30:5(V:V:V)was used as mobile phase on a C18 column,the detection wavelength was 210 nm and the flow rate was 0.6 mL/min for detection,and the external standard method was used for quantitative analysis.Results Two target compounds were separated and analyzed in 20 min.In the sample,there was a good linear relationship between the concentration of acesulfame potassium and the peak area in the range of 0.0040-0.0403 mg/mL,and the correlation coefficient was greater than 0.999.The recovery rate of acesemide was 98.58%when the standard solution with different concentrations of acesulfame potassium was added at 3 levels.There was a good linear relationship between the concentration of rebaudioside A and the peak area in the range of 0.0028-0.0283 mg/mL,and the correlation coefficient was greater than 0.999.The recovery rate of acesemide was 98.58%when the standard solution with different concentrations of rebaudioside A was added at 3 levels.All of them met the requirements of the standard GB/T 27404-2008 Laboratory quality control standard-Physical and chemical testing of food,the separation effect of target peak was better,and the efficiency is increased by 49.5%.Conclusion The proposed method is fast,accurate and sensitive,which is suitable for the simultaneous determination of acesulfame potassium and rebaudioside A in milkshake powder.