摘要
目的观察加味二陈汤对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)大鼠血脂、肝脂与血清瘦素水平的影响。方法SD大鼠(80只)随机分为4组,每组20只,正常组予普通饲料喂养,模型组、辛伐他汀组(1.8 mg/kg)、加味二陈汤组(4.5 g/kg)予高脂饲料喂养。于第9周开始灌胃给药,持续4周。分别于10、12周末观察各组大鼠体质量、肝重、肝组织HE染色、肝功能(AST)、血脂(血TG、HDL-C、LDL-C)、肝脂(肝TG)、血清瘦素水平。结果10、12周末病理切片结果显示,高脂饮食大鼠肝脏出现典型的脂肪肝病变。生化指标及ELISA结果显示,模型组肝功能、血脂、肝脂及血清瘦素水平明显升高。加味二陈汤组较模型组,肝脂、血脂水平下降,脂质沉积和脂肪肝变性程度有所改善,血清瘦素水平明显下调(P<0.05)。结论加味二陈汤可下调血清瘦素水平,下调血脂、肝脂水平,表明加味二陈汤可能通过化痰祛瘀法治疗非酒精性脂肪性肝病,而降低瘦素水平可能是其作用机制之一。
Objective To observe the effects of modified Erchen Decocion on the levels of blood lipid,liver fat and serum leptin in rats with non-alcoholic fatty liver disease.Methods 80 SD rats were randomly divided into normal control group,model group,simvastatin group(1.8 mg/kg)and modified Erchen Decoction group(4.5 g/kg),with 20 rats in each group.The normal group were fed with ordinary diet,the other groups were fed with high-fat diet.Gavage was started at the 9th week and lasted for 4 weeks.The weight,liver weight,HE staining,liver function(AST),blood lipids(blood TG,HDL-C,LDL-C),liver lipids(liver TG),serum leptin level were detected.Results At the end of 10 and 12 weeks,pathological results showed that the liver of high-fat diet rats with a typical hepatic steatosis.Biochemical and ELISA results showed,the liver function,blood lipids,liver lipids and serum leptin level in the model group were significantly increased.Compared with model group,the degree of lipid deposition and liver steatosis in modified Erchen Decoction group had obviously improved,and the level of serum leptin was significantly reduced(P<0.05).Conclusion Modified Erchen Decoction could ruduce serum leptin level,lower blood lipids and liver lipids levels.This indicated that modified Erchen Decoction could treat non-alcoholic fatty liver and the mechanism may be related to leptin reducing.
作者
杜冰
王佳宁
杨艳
洪力
闫龙腾
罗德霞
姚政
DU Bing;WANG Jianing;YANG Yan;HONG Li;LUO Dexia;YAN Longteng;YAO Zheng(Yunnan University of Chinese Medicine,Kunming 650500,China;Yulin Education Demonstration Comprehensive Practice Base,Yulin 719000,China;Yunnan Lincang Health College,Lincang 677000,China;Key Laboratory of Microcosmic Syndrome Differentiation,Education Department of Yunnan,Kunming 650500,China)
出处
《云南中医学院学报》
2020年第5期1-5,27,共6页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家自然科学基金项目(81860812)
云南省应用基础研究项目(2013FZ093)
云南省应用基础研究-中医联合专项[2017FF116(-021)]。
关键词
加味二陈汤
非酒精性脂肪性肝病
瘦素
modified Erchen Decoction
non-alcoholic fatty liver
leptin