江苏省沙门氏菌全基因组序列中外排泵耐药基因分布及其特征研究

Distribution and characteristics of efflux pumps drug-resistant genes in Salmonella spp.whole genome isolated from Jiangsu province

目的了解江苏省沙门氏菌的耐药情况及其耐药相关基因的分布状况。方法根据美国实验室标准化临床协会的操作规范推荐的肉汤稀释法测定沙门氏菌的药物敏感性,应用在线数据库分析基于多个位点的序列型、耐药基因的种类和数量。结果至少一半以上的沙门氏菌对链霉素、氨苄西林、复方磺胺甲恶唑、四环素、大观霉素、利福平耐药。尤其是对大观霉素和利福平的耐药率高达90%以上。D群沙门氏菌是主要的血清群。ST11型为主要的序列型。与外排泵耐药相关的9种MFS耐药基因均可以检出,最多的沙门氏菌携带8种基因。结论江苏沙门氏菌的耐药问题比较严重,在临床用药和畜牧业养殖中,应加强抗生素合理使用。

Objective To investigate the drug resistance of Salmonella and the distribution of drug resistance and relatedgenes in Jiangsu province.Methods According to the broth dilution method recommended by the American Laboratory Standardization Clinical Association,the drug susceptibility of Salmonella was determined.The sequence based on multiple sites,the type and number of drug resistance genes were analyzed by the online database.Results More than half of Salmonella bacteria were resistant to streptomycin,ampicillin,cotrimoxazole,tetracycline,spectinomycin,and rifampicin.Especially,the drug resistance rate to spectinomycin and rifampicin was as high as more than 90%.Salmonella group D was the main serogroup,Type ST11 was the main sequence type.All 9 MFS resistance genes related to efflux pump resistance were detected,and the most common Salmonella bacteria carried eight genes.Conclusion The drug resistance of Salmonella in Jiangsu is relatively serious.In clinical medicine and animal husbandry,the rational use of antibiotics should be strengthened.