丙烯酰胺母源性暴露对新生大鼠海马GFAP和S100β表达的影响

Effects of maternal exposure to acrylamide on the expression of GFAP and S100β in hippocampus of newborn rats

目的:探讨丙烯酰胺(ACR)母源性暴露对新生大鼠海马胶质纤维酸性蛋白(GFAP)和S100β表达的影响。方法:32只SD母鼠随机分为对照组(control)、低剂量ACR暴露组(ACR-L)、中剂量ACR暴露组(ACR-M)和高剂量ACR暴露组(ACR-H),每组8只,母鼠自妊娠第6 d起连续14 d灌胃给药,control组给予ddH20,ACR组分别给予4、8以及16 mg/kg ACR直至仔鼠出生取出海马组织,建立ACR染毒孕鼠模型。HE染色观察各组大鼠海马神经元细胞形态的改变;免疫组化和Western Blot检测海马GFAP和S100β蛋白的表达情况。结果:HE染色显示随ACR染毒剂量增大,新生大鼠海马颗粒层神经元排列疏松;与control组相比,各剂量组GFAP的表达量呈浓度依赖性减少,而S100β的表达量随染毒剂量的增大而增加。结论:ACR母源性暴露对新生大鼠海马神经元造成损伤,其机制可能与下调GFAP表达及上调S100β表达有关。

Objective: To explore the effects of maternal exposure to acrylamide( ACR) on the expression of glial fibrillary acid protein( GFAP) and S100β in the hippocampus of newborn rats. Methods: Thirty-two SD pregnant rats were randomly divided into control group,low-dose ACR group( ACR-L),medium-dose ACR group,and high-dose ACR group( ACR-H). Rats was exposed to ACR by gavage at 0,4,8,and 16 mg/kg,once per day for 14 days from embryonic 6 th day. The morphology of hippocampal neurons of rats were observed by HE staining. Expression of GFAP and S100β was detected by immunohistochemistry and Western Blot. Results: HE staining showed that neurons in the hippocampal granular layer of newborn rats were loosely arranged with increasing of the ACR dose. Compared with the control group,the expression of GFAP was decreased and the expression of S100β was significantly increased in a dose-dependent manner. Conclusion: Maternal exposure to ACR induced hippocampal neurons damage in newborn rats,and its toxicity may be related to down-regulation of GFAP and up-regulation of S100β.