海南油茶2个优良单株的比较转录组学分析

Comparative Transcriptome Analysis of Two Clones of Camellia vietnamensis in Hainan

选取“万海1号”和“万海4号”2个海南产油茶(Camellia vietnamensis)优良单株为试验材料,采用新一代高通量Illumina转录组测序技术,对二者进行转录组测序分析,并进行差异比较。结果表明,“万海1号”的特异性基因中,有651条被注释到与植株抗病性相关的途径,约占5.26%,有510条被注释到脂肪酸代谢相关的途径,约占4.12%;“万海4号”中有169条特异性基因被注释到倍半萜和三萜生物合成、油菜素类固醇生物合成、类固醇生物合成和单萜类生物合成等植物次生代谢相关途径。2个样本的单拷贝同源基因多被富集到与抗氧化能力相关的GO条目,以及与氨基酸合成代谢相关的KEGG条目,且P值均小于0.05,显示出显著的相关性。本试验可进一步探究海南本地油茶的生理特性及代谢过程,作为海南本地油茶的优良品种选育的科学依据。

In order to further explore the physiological characteristics and metabolic process and also to provide scientific basis for the selection of excellent clones of Camellia vietnamensis in Hainan,mature leaves of two years old plants from two clones Camellia vietnamensis‘Wanghai1’and‘Wanhai4’derived from two elite individuals of Camellia vietnamensis plants from Hainan were selected for transcriptome sequencing and analysis by using next-generation high-throughput Illumina transcriptome sequencing technology,and their differences in gene expression were compared.The results showed that among the specific genes of‘Wanhai1’there were 651 annotated to the path related to plant disease resistance,accounting for about 5.26%,and 510 annotated to the pathway related to fatty acid metabolism,accounting for about 4.12%,and that among the specific genes in‘Wanhai2’there were 169 annotated to pathways such as sesquiterpenoid and triterpenoid biosynthesis,brassinosteroid biosynthesis,steroid biosynthesis and monoterpenoid biosynthesis,which are related to plant secondary metabolism.The single-copy homologous genes of the two samples were mostly enriched to GO entries related to antioxidant capacity and KEGG entries related to amino acid anabolic metabolism,and their P levels were less than 0.05,showing a significant correlation between their genes and antioxidant capacity or amino acid anabolic metabolism.This might indicate that Clone‘Wanhai1’has higher stress resistance while Clone‘Wanhai4’accumulates more secondary metabolites.