摘要
CRISPR/Cas9是目前广泛应用的基因编辑技术,其操作方便、效率高、成本低。水稻作为全球粮食安全的战略作物,优质高产的水稻培育尤为重要,CRISPR/Cas9技术为水稻分子育种带来了突破。本文回顾了基因编辑技术的发展,详细介绍了CRISPR/Cas9系统的结构、组成及原理,重点阐述了该技术在水稻分子育种中的应用,探讨CRISPR/Cas9系统存在的问题以及其在分子育种的发展前景。
CRISPR/Cas9 gene-editing technology is a widely used gene-editing system,which is easy-to-use,efficient and cost-effective.Rice is a strategic crop for global food security.Thus cultivation of high quality and high-yielding rice is of great importance.The CRISPR/Cas9 technology has brought a breakthrough in rice molecular breeding.This paper reviews the development of gene-editing technology,introduces the structure,composition and principle of the CRISPR/Cas9 gene-editing system,focuses on the applications of the technology in rice molecular breeding and discusses the problems and perspectives of its applications in rice molecular breeding.
作者
刘欣欣
李赫
卜庆云
田晓杰
王臻昱
何明良
唐佳琦
李秀峰
孟威
LIU Xinxin;LI He;BU Qingyun;TIAN Xiaojie;WANG Zhenyu;HE Mingliang;TANG Jiaqi;LI Xiufeng;MENG Wei(College of Life Science,Northeast Forestry University,Harbin 150060,China;Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China;College of Life Science,Northeast Agricultural University,Harbin 150030,China)
出处
《土壤与作物》
2021年第1期18-26,共9页
Soils and Crops
基金
黑龙江省自然科学基金(面上项目)(C2018065)
国家自然科学基金青年基因(31801327)。
