饲料级鸡肉粉对黄鳝生长、消化酶及血清生化指标的影响

Effect of Fish Meal Replaced by Feed Grade Poultry By-Product Meal onGrowth Performance, Intestinal Digestive Enzyme Activities and SerumBiochemical Indices of Rice Field Eel Monopterus albus

将初始体质量(26.67±0.05) g的健康黄鳝饲养在1.5 m×2.0 m×1.5 m的网箱中,网箱内水花生覆盖水面90%以上,投喂用饲料级鸡肉粉替代鱼粉的5组等氮等脂饲料:鱼粉组(对照组)、15%替代组、30%替代组、45%替代组和60%替代组,研究以饲料级鸡肉粉替代鱼粉对黄鳝生长、肠道消化酶活力以及血清生化指标的影响。8周的养殖试验结果表明,随着替代水平的增加,黄鳝质量增加率呈下降趋势( P <0.05),当鸡肉粉替代比例超过30%时,血清补体3与补体4含量显著升高( P <0.05)。高比例替代组的饲料系数、全鱼粗脂肪和甘油三酯含量显著高于鱼粉组( P <0.05) ,30%替代组和45%替代组的低密度脂蛋白显著升高( P <0.05)。除45%替代组外,各替代组肠道脂肪酶活力均显著下降( P <0.05)。各替代组白蛋白含量均低于鱼粉组但差异不显著( P >0.05)。15%替代组、30%替代组的谷丙转氨酶活性和60%替代组的谷草转氨酶活性均显著高于鱼粉组( P <0.05)。综上所述,本试验条件下,饲料级鸡肉粉可替代饲料中30%的鱼粉而不影响黄鳝的生长和生理功能。

An 8-week feeding trial was conducted to evaluate the effect of replacing fish meal (FM) with feed grade poultry by-product meal on growth performance and physiological indicators of rice field eel Monopterus albus . In this experiment, healthy rice field eel with an initial body weight of (26.67±0.05) g were reared in a cage of 1.5 m×2.0 m×1.5 m covered with alligator weed Alternanthera philoxeroides more than 90% of the water surface, and fed 5 kinds of isotonic isoenergetic diets prepared by fish meal replacement by various levels of poultry by-product meal: fish meal group (control group), 15% substitution group (group 15%), 30% substitution group (group 30%), 45% substitution group (group 45%) and 60% substitution group (group 60%) with 3 replicates per group and 60 fish per replicate to evaluate the effect of using feed-grade poultry by-product meal instead of fish meal on rice field eel growth, intestinal digestive enzyme activity, and serum biochemical indicators. Results showed that the significant decrease in weight gain rate, and significant increase in levels of C3 and C4 were observed in the fish fed the diets with the replacement ratio of over 30% ( P <0.05). The fish fed the diets with the high replacement ratio had significantly higher food conversion ratio, and contents of crude fat and triglyceride (TG) than the fish fed the control diet did ( P <0.05), significantly higher low-density lipoprotein contents in group 30% and group 45% ( P <0.05). There was lower intestinal lipase activity in all the groups ( P < 0.05 ), except for the group 45%, without significant difference. Albumin content was found to be lower in each treatment group than that in the control group ( P >0.05). There were significantly higher GPT activities in group 15% and group 30% than that in the control group ( P <0.05). To sum up, no negative effect on the growth performance and physiological function was observed in the rice field eel fed the diet with less than 30% fish meal replaced by the feed grade poultry by-product meal under the conditions of this experiment.