类风湿关节炎中医体质及其与HLA-DRB1基因多态性的相关性研究

Investigation on TCM Constitution of Those with Rheumatoid Arthritis and Its Correlation with HLA-DRB1 Gene Polymorphism

目的:探讨类风湿关节炎患者中医体质与人类白细胞抗原(HLA)-DRB1基因多态性的关系。方法:根据《中医体质分类与判定》标准设计调查问卷,对200例类风湿关节炎患者进行中医体质类型的调查,以直接碱基序列分析基因分型技术结合特异性引物聚合酶链反应方法测定类风湿关节炎患者的HLA-DRB1基因型,运用统计学方法比较组间体质类型分布差异及组间基因频率的分布差异。结果:阳虚质、气郁质为类风湿关节炎患者易患体质类型;HLA-DRB1*04在不同体质类型患者中分布频率最高,其中阳虚质、气郁质人群HLA-DRB1*04(41.07%、40.00%)基因频率显著高于其他等位基因。结论:中医体质类型与HLA-DRB1基因多态性对类风湿关节炎发生有一定的影响,但其间的相互关系有待进一步研究明确。

Objective:To explore the relationship between TCM Constitution and HLA-DRB1 gene polymorphism in patients with rheumatoid arthritis.Methods:According to the standards of Classification and Determination of Constitution in TCM,a questionnaire was designed to investigate 200 patients with rheumatoid arthritis.HLA-DRB1 genotypes of patients with rheumatoid arthritis were determined by direct base sequence analysis genotyping technology combined with specific primer polymerase chain reaction,and the differences of constitution types distribution and gene frequency distribution between groups were compared by statistical methods.Results:Yang deficiency and qi stagnation were the predisposing constitution types of rheumatoid arthritis patients.HLA-DRB1*04 had the highest frequency in different constitution types,in which the gene frequency of HLA-DRB1*04(41.07% and 40.00%)in those with Yang deficiency and qi stagnation was significantly higher than other alleles.Conclusion:TCM constitution type and HLA-DRB1 gene polymorphism have certain influence on the occurrence of rheumatoid arthritis,but the relationship between them needs further study.