低温贮藏对草莓苗的生理变化及生长发育的影响

Low Temperature Storage: Effect on the Physiological and Developmental Change of Strawberry Ramets

为了建立草莓苗安全冷藏体系,本研究以草莓品种‘宁玉’为试材,测定了低温对草莓苗叶绿素、SOD、POD、MDA、可溶性糖、脯氨酸、内源激素含量等生理指标、物候期和产量的影响。结果表明叶绿素a、b含量及其比值、SOD值和可溶性糖含量逐渐下降,而POD值逐渐升高。处理21天时叶绿素a下降幅度为0.28 mg/g,SOD下降了46.5%,POD上升了428.73%;脯氨酸含量在处理第3天时急剧增加,之后逐渐下降;MDA含量整体呈下降趋势;GA3和IAA的含量先降低后升高,ABA含量呈现相反变化,处理8天分别达到谷值和峰值;处理8天以内草莓物候期提前、早期产量增加。5℃条件下,草莓种苗可以安全贮藏8天,达到早熟和稳产。

To establish a safe cold storage system for strawberry ramets, the strawberry variety‘Ning Yu’was used as material. The effects of low temperature on the physiological indexes such as the contents of chlorophyll, SOD, POD, MDA, soluble sugar, proline and endogenous hormones, phenological phase and yield were measured. The results showed that under the low temperature, the contents of chlorophyll a, chlorophyll b and soluble sugar, the ratio of chlorophyll a/b, SOD activity and the soluble sugar were decreased, however POD activity was increased. After the treatment of 21 days, the decreased range of chlorophyll a content was0.28 mg/g, SOD was decreased by 46.5%, while POD was increased by 428.73%. The content of proline was increased quickly on the 3rd day of treatment, and then decreased gradually. MDA content showed a descending trend as a whole. GA3 and IAA were decreased first and then increased, but the content of ABA was increased rapidly first and then decreased, reaching valley and peak respectively at the 8 th day of the treatment. In addition, within 8 days of low temperature storage, the phenological phase of strawberry was ahead of schedule,and the early-period yield was increased. In summary, under the storage condition of 5℃ low temperature, the strawberry ramets can be safely stored for 8 days, and early-mature and stable production could be achieved.