基于L-半胱氨酸的手性硅胶球固定相研究

Chiral Stationary Phase of Silica Gel Sphere Based on L-cysteine

硅胶是目前使用最广泛的高效液相色谱固定相之一,以L-半胱氨酸为手性源,利用堆砌硅珠法制备手性硅胶球,且手性来源于硅胶球自身的孔结构和骨架。由于这是一种手性的全无机硅胶球,其流动相使用宽泛,机械强度高。将其用作HPLC手性固定相,成功拆分了12种外消旋体,其中有3种外消旋体达到基线分离,此种手性硅胶球对11种苯系位置异构体有不同的分离效果,其中达到基线分离的苯系位置异构体有7种。该固定相在手性拆分领域能发挥其独特优势。

Silica gel is one of the most widely used stationary phases for high performance liquid chromatography(HPLC).The chiral silica gel spheres were prepared by stacking silica beads method with L-cysteine as the chiral source.Its chirality comes from the pore structure and skeleton of the silica gel ball itself.As it is a kind of inorganic silicone ball,many mobile phases can be used with good mechanical strength.When it was used as chiral stationary phase in HPLC,twelve racemes were successfully separated,among which three achieved baseline separation.This chiral silica gel ball had different separation effects for eleven benzene position isomers,among which seven reached baseline separation.The stationary phase can exert its unique advantages in the field of chiral resolution.