基于RNA-Seq分析MpigE在红曲霉色素生物合成中的转录调控

Unraveling MpigE involved in pigment biosynthesis in Monascus purpureus Mp-21 by RNA-Seq transcriptome profiling analyses

【目的】分析MpigE的缺失在转录水平对红曲色素产生的影响。【方法】对实验室保存的野生型紫色红曲霉(Monascus purpureus)Mp-21和△MpigE菌株进行高通量转录组测序、注释、表达差异基因功能富集分析和基因通路富集分析,在转录水平揭示MpigE缺失后红曲霉色素产量变化的原因。【结果】通过RNA-Seq测序,每个样品获得7.5–8.5 Gb的原始数据,经过拼接后得到7219个转录本(Unigenes),其中成功注释的为5692个。差异基因表达富集分析发现基因缺失菌株△MpigE相较于野生型菌株Mp-21上调差异基因达到199个,下调差异基因为293个。【结论】MpigE的缺失能够促进红曲霉中中央碳代谢和乙酰辅酶A代谢相关基因的表达以此影响色素生物合成。

[Objective]To analyze the effect of MpigE(one of Monascus purpureus genes)deletion on the transcription of Monascus pigments.[Methods]The wild-type Monascus purpureus Mp-21 and the MpigE were analyzed by high-throughput transcriptome sequencing,annotation,enrichment of gene function analysis and gene expression differences pathway enrichment analysis.The transcription level revealed the reason for the change of pigment production after MpigE deletion.[Results]By RNA-seq sequencing,7.5–8.5 Gb of original data were obtained from each sample,and 7219 Unigenes were obtained after de novo assembly,among which 5692 were successfully annotated.The enrichment analysis of differentially expressed genes showed that compared with the wild-type strain of Mp-21,MpigE had 199 up-regulated differentially expressed genes and 293 down-regulated differentially expressed genes.[Conclusion]The deletion of MpigE can affect the biosynthesis of pigment by promoting the expression of central carbon metabolism and acetyl-CoA metabolism-related genes in Monascus.