超声引导股动脉穿刺置管在血管内介入方法制备恒河猴急性脑梗死模型中的应用

Application of ultrasound-guided femoral artery puncture and catheterization on endovascular ischemic stroke models of adult rhesus monkeys

目的探讨超声引导股动脉穿刺置管在血管内介入方法制备恒河猴急性脑梗死模型中的应用效果。方法选择拟行大脑中动脉M2段栓塞建模的健康成年恒河猴16只,雄性,采用数字表法随机分为2组(n=8):对照组(C组)、超声引导组(U组)。所有恒河猴采用0.1 mL/kg氯胺酮及速眠新的混合液肌内注射进行基础麻醉,待动物入睡后行气管内插管,接呼吸机行机械通气,麻醉维持采用持续输注丙泊酚(5~8 mg·kg^(-1)·h-1)。C组采用传统指尖触摸法进行股动脉穿刺置管,U组采用超声引导平面内-平面外联合法进行股动脉穿刺置管。比较两组1次、2次穿刺置管成功率、总成功率、穿刺置管次数、穿刺置管花费时间及并发症发生率。结果C组与U组1次穿刺置管成功率分别为25.0%和62.5%,2次穿刺置管成功率分别为50.0%和87.5%、总成功率分别为75.0%和100%。C组与U组穿刺置管次数分别为(3.0±1.6)次和(1.5±0.8)次,穿刺置管花费时间分别为(15.8±6.1)min和(5.4±2.9)min。与C组比较,U组穿刺置管次数少,穿刺置管花费时间较短,两组差异有统计学意义(P<0.05);与C组比较,U组1次、2次穿刺置管成功率及总成功率均较高,但差异无统计学意义(P>0.05)。两组股动脉穿刺置管并发症发生率比较差异无统计学意义(P>0.05)。结论在血管内介入方法制备恒河猴急性脑梗死模型中,采用平面内-平面外联合技术进行超声引导股动脉穿刺,可提高首次穿刺置管成功率及总成功率,减少穿刺置管次数,缩短穿刺置管花费时间。

Objective Investigate the effect of ultrasound-guided femoral artery puncture and catheterization in the development of ischemic stroke models of adult rhesus monkeys through endovascular methods.Methods Sixteen male rhesus monkeys were randomized into 2 groups:control group(Group C)and ultrasound-guided group(Group U).Su-mian-xin injection combined with 0.1 mL/kg ketamine was given before endotracheal intubation.After intubation,the animals received mechanical ventilation.Anesthesia was maintained with propofol infusion(5-8 mg·kg^(-1)·h-1)in both groups.Classic palpation method was used for femoral artery puncture and catheterization in Group C.Ultrasound-guidance combined in-plane and out-of-plane was used for femoral artery puncture and catheterization in Group U.The first attempt success rate,second attempt success rate,total success rate,numbers of attempts,period of puncture and catheterization time and incidence of complications were compared between two groups.Results The frequency of attempts in Group U was less than that in Group C(P<0.05).Compared with Group C,the mean time to success was shorter in Group U(P<0.05).The success rate of the first and second attempt and total success rates were high in Group U,with no statistical differences(P>0.05).There was no difference in the incidence of complications between the two groups(P>0.05).Conclusion Ultrasound-guidance combined in-plane and out-of-plane used for femoral artery puncture and catheterization improved the initial attempt success rate and total success rate,reduce the frequency of attempts and the mean time on endovascular ischemic stroke models of adult rhesus monkeys.