摘要
The application of traditional Chinese medicine(TCM)has made great contributions to the fight against the epidemic of coronavirus disease-2019(COVID-19).Despite the remarkable therapeutic effects of TCM,the molecular mechanisms of TCM formulae inhibiting COVID-19 are still not fully understood.Here,we cφm-bined the automated high throughput sequencing-based high throughput screening(HTS^(2))assay with bioinformatics and computer-aided drug design(CADD)to investigate the molecular mechanisms of TCM-mediated therapeutic effects on COVID-19-related cytokine storm(Fig.1a).
中药抑制新冠肺炎相关细胞因子风暴的分子机制尚不清晰.本文首先通过HTS2技术,在基因表达层面上,大规模检测了578味中药提取物对与细胞因子风暴紧密相关的信号通路的调控作用,发现53味中药对这些通路具有显著抑制作用;评估了国家及地方新冠肺炎诊疗方案中出现的338个方剂,发现理中汤、六君子汤、黄连解毒汤、清肺排毒汤在抑制细胞因子风暴方面排在前列;鉴别了相关中药的潜在活性成分,进一步揭示了中药抑制新冠肺炎相关细胞因子风暴的化学基础.本研究系统探究了中药调节新冠相关细胞因子风暴的分子机制,为中药治疗新冠肺炎药效机制的解析提供了科学依据.
作者
Yifei Dai
Weijie Qiang
Yu Gui
Xue Tan
Tianli Pei
Kequan Lin
Siwei Cai
Liang Sun
Guochen Ning
Jianxun Wang
Hongyan Guo
Yimin Sun
Jing Cheng
Lan Xie
Xun Lan
Dong Wang
戴逸飞;强伟杰;贵昱;谭雪;裴天丽;林克权;蔡思唯;孙亮;宁国琛;王建勋;郭弘妍;孙义民;程京;谢兰;蓝勋;王栋(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Basic Medical Sciences,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Department of Basic Medical Sciences,School of Medicine,Tsinghua University,Beijing 100084,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;High Performance Computing Department,National Supercomputing Center in Shenzhen,Shenzhen 518055,China;Department of Electronic and Computer Engineering,College of Engineering,Drexel University,Philadelphia 19104,USA;Dongli District Jinqiao Street Community Health Service Center,Tianjin 300300,China;Department of Biomedical Engineering,School of Medicine,Tsinghua University,Beijing 100084,China;School of Life Sciences,Beijing University of Chinese Medicine,Beijing 100029,China;National Engineering Research Center for Beijing Biochip Technology,Beijing 102206,China;State Key Laboratory of Membrane Biology,School of Medicine,Tsinghua University,Beijing 100084,China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Hangzhou 310003,China;Medical Systems Biology Research Center,School of Medicine,Tsinghua University,Beijing 100084,China)
基金
supported by the National Natural Science Foundation of China(81673460)
Sichuan Youth Science and Technology Innovation Research Team of Experimental Formulology(2020JDTD0022)。
