超声波-酶法提取玉竹多糖及其抗疲劳泡腾片研制

Ultrasonic-enzymatic Extraction Polygonatum Odoratum Polysaccharide and Preparation of Its Anti-fatigue Effervescent Tablets

本试验采用超声波-酶法提取玉竹多糖,将提取的多糖制备具有抗疲劳功能的泡腾片。进行单因素试验和正交试验确定最佳提取条件,结果表明:当超声时间30 min,超声温度30℃,液料比30∶1,纤维素酶添加量2.2%时提取的粗多糖最多,提取率达6.13%。利用提取的多糖搭配安赛蜜、泡腾剂和其他辅料等,研制玉竹多糖抗疲劳泡腾片。最优配方为玉竹多糖添加量25%,柠檬酸与碳酸氢钠的比例1.0∶1,安赛蜜添加量6%,所制成的泡腾片经试验表明具有一定的抗疲劳效果,能明显降低小鼠机体血清尿素氮含量。

Ultrasonic-enzymatic extraction of Polygonatum odoratum polysaccharide was used in this study. The extracted polysaccharide was prepared into effervescent tablets with anti-fatigue function. Single factor test and orthogonal test were carried out to determine the best extraction conditions. The results showed that the optimal conditions were ultrasonic time 30 min, ultrasonic temperature 30 ℃, liquid to solid ratio 30∶1, cellulase content 2.2%, and the extraction rate of crude polysaccharides was up to 6.13%. Using the extracted polysaccharide with acesulfame potassium, effervescent and other auxiliary materials, the anti-fatigue effervescent tablets with Polygonatum odoratum polysaccharide were prepared. The optimal formula is 25% Polygonatum odoratum polysaccharide, the ratio of citric acid to sodium bicarbonate is 1.0∶1, and acesulfame potassium is 6%. The experimental results show that the effervescent tablet has a certain anti-fatigue effect, and can significantly reduce the content of serum urea nitrogen in mice.