卵巢上皮癌组织中circFNDC3B与miR-99a的表达

Expression of circFNDC3B and miR-99a in epithelial ovarian carcinoma

目的检测卵巢上皮癌组织中circFNDC3B及miR-99a的表达,分析其与临床病理特征之间的关系及在卵巢上皮癌诊治中的应用价值。方法选取2019年8月~2020年8月于华北理工大学附属唐山工人医院妇产科手术切除的42例卵巢上皮癌组织标本(卵巢上皮癌组),同期良性卵巢肿瘤30例(良性肿瘤组)和正常卵巢组织30例(正常卵巢组)作为对照,应用实时荧光定量反转录聚合酶链反应法(PCR)检测各组卵巢组织中circFNDC3B及miR-99a的表达,并应用统计学软件分析其与临床病理特征之间的关系及二者在卵巢上皮癌组织中表达的相关性。结果circFNDC3B、miR-99a在卵巢上皮癌组中的表达较良性肿瘤组和正常卵巢组均降低,差异具有统计学意义(P<0.05)。卵巢上皮癌组织中circFNDC3B的表达与CA125、淋巴结转移、腹膜转移、FIGO分期以及病理分化显著相关(P<0.05),不同年龄、肿瘤直径大小、组织学类型及有无腹腔积液的患者circFNDC3B相对表达量差异均无统计学意义(P>0.05)。mir-99a的表达与CA125、淋巴结转移、腹膜转移、FIGO分期、病理分化和腹腔积液显著相关(P<0.05),不同年龄、肿瘤直径大小、组织学类型患者mir-99a相对表达量的差异均无统计学意义(P>0.05)。Pearson相关性分析显示circFNDC3B和miR-99a表达呈正相关(r=0.923,P<0.001)。结论卵巢上皮癌组织中circFNDC3B及miR-99a的表达均降低,且与临床特征密切相关,可能共同参与卵巢上皮癌的发生发展过程。

Objective To investigate the expression of circFNDC3B and miR-99a in epithelial ovarian cancer and analyze the relationship between circFNDC3B and miR-99a with clinic opathological features of epithelial ovarian cancer for exploring the applicatal value of circFNDC3B and miR-99a in the diagnosis and treatment of epithelial ovarian cancer.Methods From August 2019 to August 2020,42 cases of epithelial ovarian cancer tissues(epithelial ovarian group),30 cases of benign ovarian tumor(benign tumor group)and 30 cases of normal ovarian tissue(normal ovarian group)in Tangshan Gongren Hospital were selected.Real time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detectcirc circFNDC3B and miR-99a in ovarian tissues of each group.Then the relationship between the expression of circFNDC3B,miR-99a and the clinic opathological characteristics of patients were analyzed by statistical software.Results The expression of circFNDC3B and miR-99a in epithelial ovarian group was significantly lower than that in benign tumor group and normal ovarian group(P<0.05).The expression of circFNDC3B was correlated with CA125,lymph node metastasis,peritoneal metastasis,FIGO stage and pathological differentiation(P<0.05).There was no significant difference in the relative expression of circFNDC3B among age,tumor diameter,histological type and ascites(P>0.05).The expression of miR-99a was significantly ralated to CA125,lymph node metastasis,peritoneal metastasis,FIGO stage,pathological differentiation and ascites(P<0.05).There was no significant difference in the expression of miR-99a among patients with different age,tumor diameter and histological type(P>0.05).The expression of circFNDC3B was positively correlated with miR-99a(r=0.923,P<0.001).Conclusion The expressions of circFNDC3B and miR-99a in epithelial ovarian cancer are all decreased,which are closely related to clinical features,and may play an important role in the development of epithelial ovarian cancer.