肌萎缩侧索硬化患者血清SOD、GSH-Px、MIP-1α、VEGF水平与肌电图特征及病程的关系研究

Relationship Study between Serum SOD,GSH-PX,MIP-1α and VEGF Levels and Electromyogram Characteristics and Disease Course in Patients with Amyotrophic Lateral Sclerosis

目的:研究肌萎缩侧索硬化(ALS)患者血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、巨噬细胞炎性蛋白-1α(MIP-1α)、血管内皮生长因子(VEGF)水平与肌电图特征及病程的关系。方法:将从2018年12月起直至2020年12月,我院收治的ALS患者86例纳入研究,记作病变组。另取同期90例于我院进行体检的健康人员作为对照组。检测并比较两组血清SOD、GSH-Px、MIP-1α、VEGF水平及肌电图特征。将所有病变组患者根据病程的差异分为病程较长组42例以及病程较短组44例,比较两组血清SOD、GSH-Px、MIP-1α、VEGF水平以及肌萎缩侧索硬化症功能评分量表(ALSFRS-r)评分。采用Pearson相关性分析ALS患者血清SOD、GSH-Px、MIP-1α、VEGF水平与肌电图特征及病程的关系。结果:病变组血清SOD、GSH-Px水平均低于对照组,而MIP-1α、VEGF水平均高于对照组(均P<0.05)。病变组各项肌电图参数水平均低于对照组(均P<0.05)。病程较长组血清SOD、GSH-Px水平以及ALSFRS-r评分均低于病程较短组,而MIP-1α、VEGF水平均高于病程较短组(均P<0.05)。经Pearson相关性分析可得:ALS患者血清SOD、GSH-Px水平与肌电图各神经符合肌肉动作电位(CMAP)、ALSFRS-r评分均呈正相关,与病程呈负相关(均P<0.05);MIP-1α、VEGF水平则与肌电图各神经CMAP、ALSFRS-r评分均呈负相关,与病程呈正相关(均P<0.05)。结论:ALS患者血清SOD、GSH-Px水平较低,MIP-1α、VEGF水平较高,且和肌电图特征以及病程密切相关,值得临床关注。

Objective:To study the relationship between serum superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),macrophage inflammatory protein-1α(MIP-1α),vascular endothelial growth factor(VEGF)levels and electromyogram characteristics and disease course in patients with amyotrophic lateral sclerosis(ALS).Methods:From December 2018 to December 2020,86 patients with ALS admitted to our hospital were included in the study,and were labeled as the pathological group.In addition,90 healthy people who underwent physical examination in our hospital during the same period were selected as the control group.Serum SOD,GSH-Px,MIP-1α,VEGF levels and electromyogram characteristics were measured and compared between the two groups.In addition,according to the difference in disease course,all patients in the pathological group were divided into 42 patients in the longer course group and 44 patients in the lower course group.Serum SOD,GSH-Px,MIP-1α,VEGF levels and amyotrophic lateral sclerosis functional rating scale revised(ALSFRS-r)score were compared between the two groups.Pearson correlation analysis was used to determine the relationship between serum SOD,GSH-Px,MIP-1α and VEGF levels and electromyogram characteristics and disease course in patients with ALS.Results:Serum SOD and GSH-Px levels in the pathological group were lower than those in the control group,while the MIP-1α and VEGF levels were higher than those in the control group(all P<0.05).All electromyogram parameters in the pathological group were lower than those in the control group(all P<0.05).The serum SOD,GSH-Px levels and ALFRS-r in the longer course group were lower than those in the lower course group,and the MIP-1α and VEGF levels were higher than those in the lower course group(all P<0.05).Pearson correlation analysis showed that the serum SOD and GSH-PX levels of patients with ALS were positively correlated with each nerve consistent with muscle action potential(CMAP)of electromyogram and ALFRS-r score,and negatively correlated with the disease course(all P<0.05).The MIP-1α and VEGF levels were negatively correlated with each nerve CMAP of electromyogram,and positively correlated with the disease course(all P<0.05).Conclusion:Serum SOD and GSH-Px of patients with ALS are lower,while MIP-1α and VEGF are higher,which are closely related to electromyogram characteristics and disease course,and it worthy of clinical attention.