阿魏酸和那他霉素对采后黑莓果实细胞壁代谢的影响

Effects of Ferulic Acid and Natamycin on Cell Wall Metabolism of Postharvest Blackberry Fruits

以黑莓品种‘Hull’为试材,采用阿魏酸和那他霉素浸泡黑莓果实,测定了果实细胞壁成分和细胞壁降解酶的活性,研究了采后处理对黑莓果实细胞壁的作用,以期阐明采后处理提高黑莓果实硬度的作用机制。结果表明:阿魏酸和那他霉素处理后果实中纤维素和果胶含量高于对照组。阿魏酸处理可显著降低α-L-阿拉伯呋喃糖苷酶(α-L-Af)活性;那他霉素处理可显著降低β-半乳糖苷酶(β-Gal)和β-甘露聚糖酶(β-Man)活性;阿魏酸和那他霉素联用可以显著降低多聚半乳糖醛酸酶(PG)、果胶甲酯酶(PME)和纤维素酶(Cx)活性,提高木葡聚糖内糖基转移酶(XET)活性。综上所述,阿魏酸和那他霉素处理可以下调黑莓果实中的细胞壁降解酶活性,提高细胞壁保护酶活性,抑制果胶、半纤维素和纤维素的分解,从而延缓黑莓果实软化。

Blackberry ‘Hull’ was used as material.The fruits of blackberry were dipped in ferulic acid and natamycin to determine cell wall components and the activities of cell wall degrading enzymes.The effect of postharvest treatment on cell wall of blackberry fruit was studied to clarify the mechanism of postharvest treatment on blackberry fruit firmness.The results showed that,the cellulose and pectin contents in blackberry fruits with the use of ferulic acid and natamycin were higher than the control.The activity of α-L-arabinofuranosidase(α-L-Af) in blackberry fruits was significantly decreased with the ferulic acid treatment.Natamycin treatment significantly decreased cell wall-degrading enzymes activities,including β-D-galaetosidase(β-Gal) and endo-1,4-β-mannanase(β-Man).The combined treatment of ferulic acid and natamycin significantly decreased cell wall degrading enzymes activities,including polygalacturonase(PG),pectin methylesterase(PME) and cellulase(Cx) and increased xyloglucan endotransglucosylase(XET).Therefore,the treatments of ferulic acid and natamycin downregulated the activity of cell wall degrading enzymes and enhanced the activity of cell wall protective enzymes,inhibited the decomposition of pectin,hemicellulose and cellulose,which resulted in delayed fruit softening.