不同炮制品组方的独活寄生汤治疗腰椎间盘突出症的实验研究

Experimental Study on the Treatment of Lumbar Disc Herniation with DuHuo JiSheng Decoction with Different Processed Products

目的比较不同炮制品组方的独活寄生汤对大鼠化学性神经根炎模型,模拟腰椎间盘突出症的镇痛、抗炎作用。方法将大鼠随机分为正常对照组、模型组或阿司匹林组、不同炮制品组方的独活寄生汤1、2、3、4、5组,在不同时间观察不同炮制品组方的独活寄生汤各组对化学性神经根炎模型的神经根周围组织中炎症介质水平、血液流变性及大鼠左后肢机械刺激痛阈值影响;将小鼠分成正常对照组、阿司匹林阳性对照组、独活寄生汤1、2、3、4、5组,以热板法研究不同炮制品组成的独活寄生汤的镇痛作用。结果与模型组比较,各治疗组中3组即由杜仲盐炙、牛膝、川芎、当归、白芍等药的酒炙品组方的独活寄生汤能显著地降低全血高切、血浆粘度,降低局部组织中前列腺素E 2、5-羟色胺含量(P<0.01),能显著升高术后大鼠机械刺激痛阈值(P<0.01),与正常对照组比较,不同炮制品组方的独活寄生汤3组能显著升高小鼠热传导引起拟痛反应痛阈值(P<0.01)。结论独活寄生汤在临床上用于治疗腰椎间盘突出症时,组方时杜仲用盐制品,牛膝、川芎、当归、白芍用酒炙品。其作用机制是通过抗炎、镇痛、改善血液循环对腰椎间盘起综合治疗作用的。

OBJECTIVE To compare the analgesic and anti-inflammatory effects of different processed DuHuo JiSheng Decoction on the rat model of chemical radiculitis.METHODS The rats were randomly divided into normal control groups,Model group or aspirin group,Groups 1,2,3,4 and 5 of DuHuo JiSheng Decoction with different processed products,the levels of inflammatory mediators in the tissues around the nerve roots of the chemical neuradiculitis model were observed in different time groups of Duhuo JiSheng decoction with different processed products,influence of hemorrheology and pain threshold of left hind limb mechanical stimulation in rats;Mice were divided into a normal control group,Aspirin positive control group,Groups 1,2,3,4,5,the analgesic effect of Duhuo Parasitic soup composed of different processed products was studied by hot plate method.RESULTS Compared with the model group,the Duhuo JiSheng decoction made from processed eucommia ulmoides salt,radix Achyranae,Ligusticum chuanxiong,Angelica angelica and Radix Paeoniae alba in the three treatment groups could significantly reduce the whole blood hypertomy and plasma viscosity in the model group.The content of prostaglandin E 2 and 5-hydroxytryptamine in local tissues was decreased(P<0.01),it can significantly increase the pain threshold of mechanical stimulation(P<0.01),Compared with the normal control group,DuHuo JiSheng decoction of different processed products can significantly increase the pain threshold of pseudo-pain response caused by heat conduction in mice(P<0.01).CONCLUSION When DuHuo JiSheng decoction is used to treat lumbar disc herniation in clinic,Eucommia is made of salt products,while Achyranthes bidentata,Ligusticum chuanxiong,angelica and paeony are made of wine.Its mechanism of action is through anti-inflammatory,analgesic,improve blood circulation on the lumbar disc comprehensive treatment.