多肽药物制备工艺研究进展

Progress on pharmaceutical engineering of peptide-based drugs

多肽是由氨基酸缩合连接而成的具有一定生物活性的化合物,在医药领域具有广阔的应用前景。多肽药物是现代医药研究的前沿方向,具有重要的社会价值和经济价值。综述了多肽生物合成法(天然原料提取法、酶解法、发酵法、基因重组法)和化学合成方法(液相合成法和固相合成法)的研究进展,重点综述化学合成法中的固相多肽合成工艺,介绍了反相高效液相色谱法、毛细管电泳法、离子交换色谱法、凝胶过滤色谱法、亲和层析法在多肽分离纯化中的应用情况,最后对多肽制药技术的未来发展进行展望。

Peptide is composed of amino acid through dehydration condensation,which is a compound molecule that has specific biological activity,and thus peptide-based drugs became an important direction of pharmaceutical engineering.Peptide drugs are the frontier of modern medical research and have important social and economic values.This paper reviews the research progress of peptide biosynthesis methods(natural raw material extraction,enzymatic solution,fermentation,gene recombination)and chemical synthesis methods(liquid-phase peptide synthesis and solid-phase peptide synthesis),focusing on the solid-phase peptide synthesis among chemical synthesis methods.This paper also introduces the application of reverse-phase high-performance chromatography,capillary electrophoresis,and ion exchange chromatography,gel filtration chromatography,and affinity chromatography.Finally,the future development of peptide pharmaceutical technology is prospected.