大肠杆菌表达病毒样颗粒的研究进展

Research progress of E.coli expressing virus-like particle vaccines

病毒样颗粒是由结构蛋白组装而成,形态结构与天然病毒高度相似的一种多聚蛋白纳米结构,但不包含任何遗传物质,因此是一种理想的疫苗形式。大肠杆菌表达系统因具有良好的安全性、生产周期短、易于放大培养等优点,被广泛应用于基因工程药物的生产,在病毒样颗粒疫苗应用上具有良好前景。本文综述了利用大肠杆菌系统表达的病毒样颗粒及其组装、表位结构特征和临床试验结果等研究进展,以期为病毒样颗粒疫苗的生产提供可靠的数据支持和设计思路。

Virus-like particles are nanostructures assembled from viral structural proteins which mimic viral characteristics but are without any genetic materials and could work as candidate vaccines.The E.coli expression system offers a convenient and effective platform for genetically engineered products,due to its safety and easy handling and could be used as a model system for virus-like particles vaccines production.This paper reviews and discuses up-to-date research progress on virus-like particles expressed in E.coli models,their assembly,epitopes structure characteristics and clinical trials.Finally,it also provides an insight into the production of virus-like particles vaccines by analyzing available data and strategies.