NAT2基因多态性与肾癌发病风险关系的病例对照研究

The relationship between polymorphism NAT2 and the risk of renal cancer in case-control study

目的:探讨NAT2等位基因M3与肾癌遗传易感性之间的关系。方法:采用1∶1配对的病例对照研究方法,病例组来自内蒙古医科大学附属医院和内蒙古自治区人民医院经确诊为肾癌的病人,对照组为同期在两家医院体检中心体检的未患泌尿系肿瘤及相关疾病人员。采用自行设计的统一调查表,对肾癌病人进行问卷调查,收集其一般情况、生活习惯等资料。应用条件Logistic回归对研究对象进行分析。利用限制性内切酶片段长度多态性的方法检测病例组和对照组NAT2基因rs1799931位点的多态性情况。结果:NAT2基因的rs1799931位点,分布在病例组和对照组之间的基因型频率(P=0.016)在两组间差异有统计学意义。而等位基因频率在两组间差异没有统计学意义(P=0.528)。条件Logistic回归分析,NAT2基因rs1799931位点多态性与肾癌无关,不同基因型的人群发生肾癌的风险差别无统计学意义。饮酒为肾癌的危险因素。结论:根据本次研究结果尚不能认为NAT2基因rs1799931位点多态性与肾癌发病相关。

Objective:To investigate the relationship between NAT2 allele M3 and genetic susceptibility to renal cancer.Methods:A 1∶1 case-control study was conducted.The case group was from the Affiliated Hospital of Inner Mongolia Medical University and the People’s Hospital of Inner Mongolia Autonomous Region.A uniform questionnaire designed by ourselves was used to investigate the patients with renal cancer and collect their general conditions,live habits and other data.Conditional Logistic regression was used to analyze the research objects.Polymorphism of NAT2 gene rs1799931 in the case group and the control group was detected by using restriction endonuclease fragment length polymorphism.Results:The genotype frequency(P=0.016)of the rs1799931 site of NAT2 gene between the case group and the control group was significantly different between the two groups.However,there was no significant difference in allele frequency between the two groups(P=0.528).Conditional Logistic regression analysis showed that polymorphism at rs1799931 of NAT2 gene was not associated with renal cancer,and there was no statistically significant difference in the risk of renal cancer among people with different genotypes.Alcohol consumption is a risk factor for kidney cancer.Conclusion:Based on the results of this study,polymorphism at rs1799931 of NAT2 gene is not associated with renal cancer.