一种功能食品增强顺铂抗肿瘤疗效研究

A functional food enhances the anti-tumor efficacy of cisplatin

目的探索一种功能食品(五神)的抗肿瘤作用,并探讨该功能食品在抗肿瘤治疗中的应用前景。方法取对数生长期Lewis细胞(鼠源性肺腺癌细胞系)悬液200μl(1×10^(7)/ml)接种于小鼠右后肢背部皮下构建肺癌Lewis皮下移植瘤模型。将小鼠分为以下四组:标准饮食饲养的对照组和顺铂组,添加功能食品饲料饲养的功能食品组及功能食品顺铂组,每组8只小鼠,顺铂组及功能食品顺铂组隔天按4 mg/kg体重腹腔注射顺铂,对照组与功能食品组注射等量生理盐水。实验过程中每周测量小鼠体重及移植瘤体积,按V=ab^(2)π/6(a为最大直径,b为最小直径)计算,实验终点对瘤体称重,检测小鼠血清白蛋白、后肢肌围等营养指标,通过流式细胞仪检测小鼠脾脏、血清和肿瘤组织中相关免疫细胞亚群,ELISA检测试剂盒检测IL-6^(+)T细胞及IL-10^(+)T细胞等相关细胞因子,通过免疫组织化学检测瘤组织中微血管形成情况。结果与对照组相比,功能食品组小鼠白蛋白及后肢肌围明显升高,但移植瘤体积及质量差异无统计学意义;与顺铂组相比,功能食品顺铂组移植瘤体积及质量明显降低,荷瘤小鼠免疫效应细胞亚群CD3^(+)、CD4^(+)T细胞,CD3^(+)、CD8^(+)T细胞,NK细胞的比例明显增高且差异有统计学意义;CD8^(+)IFN‐γ^(+)、CD8^(+)Perforin^(+)T细胞和IL‐10^(+)T细胞比例降低,脾脏中抑制性免疫细胞亚群MDSCs和Treg细胞及肿瘤组织中的Treg细胞降低,但差异均无统计学意义。各组IL-6及IL-10差异无统计学意义。结论功能食品能够增强顺铂的抗肿瘤疗效,这可能与改善小鼠营养状况、调节免疫等作用有关。

Objective To explore the anti-tumor effect of a functional food and discuss the application prospect of functional food in tumor.Methods Lewis cells(murine lung adenocarcinoma line)suspension of 200μl(1×10^(7)/ml)at logarithmic growth stage was inoculated subcutaneously at the back of right hind limb of mice to construct Lewis subcutaneous tumor model of lung cancer.Mice were divided into the following four groups:control group and cisplatin group fed on standard diet,functional food group and functional food cisplatin group,8 mice in each group,cisplatin group and functional food cisplatin group were intraperitoneal injected with cisplatin at 4 mg/kg body weight every other day,control group and functional food cisplatin group were injected with the same amount of normal saline.Experiments in the process of measuring weight and transplanted tumors in mice product every week,according to V=ab^(2)π/6(a for maximum diameter,b for minimum diameter)calculation,experimental destination for tumors had weighed and detection of serum albumin in mice,hind leg muscle circumference nutrition indicators,such as cells through the detection of mice spleen,related to the immune cells in serum and tumor tissue,ELISA kits IL-6^(+)and IL-10^(+)related cytokines,such as by immunohistochemical detection of tumor tissue microvascular formation.Results Compared with the control group,albumin and hindlimb muscle circumference were significantly increased in functional food group,but there was no difference in tumor volume and mass.Compared with the cisplatin group,the tumor volume and mass of the cisplatin group were significantly decreased,and the proportion of CD3^(+)CD4^(+)T cells,CD3^(+)CD8^(+)T cells and NK cells of tumor-bearing mice was significantly increased,with statistically significant differences.There was a decrease in the proportion of CD8^(+)IFN-γ^(+),CD8^(+)perforin^(+)T cells and IL-10^(+)cell,and a decrease in the subsets of inhibitory immune cells in the spleen,MDSCs and Treg,and in tumor tissues,but the differences were not statistically significant.There was no significant difference in IL-6^(+)cell and IL-10^(+)cell in each group.Conclusion Functional food can enhance the anti-tumor effect of cisplatin,which may be related to improving the nutritional status and regulating immunity of mice.