气相色谱法测定食用油中的植物甾醇

Determination of phytosterols in edible oil by GC

对食用油进行皂化前处理,以胆甾烷醇为内标,采用气相色谱法测定菜籽甾醇、菜油甾醇、豆甾醇和β-谷甾醇4种植物甾醇含量。结果表明:该方法测定食用油中植物甾醇的专一性强,方法的检出限和定量限分别为3.00~3.30 mg/100 g和9.00~11.00 mg/100 g,精密度(RSD)为0.89%,回收率为90.51%~110.25%。该方法用于测定食用油中4种植物甾醇有效可靠。

The edible oil was saponified, and the contents of brassicasterol, campesterol, stigmasterol and β-sitosterol were determined by GC method with cholesterol as internal standard. The results showed that determination of phytosterols in edible oil by GC-internal standard method was highly specific. The limits of detection and quantitation were 3.00-3.30 mg/100 g and 9.00-11.00 mg/100 g, respectively. The precision and recovery rate were 0.89% and 90.51%-110.25%, respectively. The method was reliable on analysing of four phytosterols in edible oil.