lncRNA SNHG15-210通过上调CDKN1A对宫颈癌SIHA细胞增殖的影响

Effect of lncRNA SNHG15-210 on the proliferation of cervical cancer SIHA cells by up-regulating CDKN1A

目的探讨长链非编码RNA(lncRNA)SNHG15-210对宫颈癌SIHA细胞增殖的影响及分子机制。方法采用脂质体转染技术分别将pcDNA-NC(NC组)和pcDNA-SNHG15-210(SNHG 15-210组)转染入宫颈癌SIHA细胞。实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测转染效率。CCK-8法检测SNHG15-210对细胞吸光度的影响。集落形成实验检测SNHG15-210对细胞集落形成的影响。qRT-PCR检测细胞周期蛋白依赖蛋白激酶抑制剂1A(cyclin dependent kinase inhibitor 1A,CDKN1A)信使RNA(mRNA)的表达水平。Western blot检测CDKN1A蛋白和增殖相关蛋白的表达水平。结果SNHG15-210组和NC组SIHA细胞中SNHG15-210表达分别为(1.14±0.37)和(11.69±2.62),NC组SNHG15-210表达明显少于SNHG15-210组(t=3.99,P<0.01)。SNHG15-210过表达可显著抑制SIHA细胞的增殖(P<0.05)。与NC组相比,SNHG15-210组细胞的集落形成数明显减少(P<0.05)。与NC组相比,SNHG15-210组SIHA细胞中CDKN1A蛋白表达明显增加,Cyclin D1和Cyclin D2蛋白表达明显降低。结论过表达SNHG15-210通过上调CDKN1A基因表达抑制宫颈癌SIHA细胞的增殖,SNHG15-210是宫颈癌潜在的分子靶点。

Objective To explore the effect of long non-coding RNA(lncRNA)SNHG15-210 on the proliferation of cervical cancer SIHA cells and its molecular mechanism.Methods By using liposome transfection technology,pcDNA-NC(NC group)and pcDNA-SNHG15-210(SNHG15-210 group)were respectively transfected into cervical cancer SIHA cells.Real-time fluorescent quantitative reverse transcriptase polymerase chain reaction(qRT-PCR)was used to detect the transfection efficiency.The CCK-8 method was used to detect the effect of SNHG15-210 on the optical density of cells.The colony formation experiment was used to detect the effect of SNHG15-210 on the cell colony formation.qRT-PCR was used to detect the expression level of cyclin dependent kinase inhibitor 1A(CDKN1A)messenger RNA(mRNA).Western blot was used to detect the expression levels of CDKN1A protein and proliferation-related proteins.Results The expressions of SNHG15-210 of SIHA cells in the NC group and the SNHG15-210 group were(1.14±0.37)and(11.69±2.62),respectively,and the expression of SNHG15-210 in the NC group was significantly lower than that in the SNHG15-210 group(t=3.99,P<0.01).Overexpression of SNHG15-210 significantly inhibited the proliferation of SIHA cells(P<0.05).Compared with that in the NC group,the number of formed colonies in the SNHG15-210 group was significantly reduced(P<0.05).Compared with those in the NC group,the CDKN1A protein expression of SIHA cells in the SNHG15-210 group significantly increased,and the expressions of Cyclin D1 and Cyclin D2 proteins significantly decreased.Conclusions Overexpression of SNHG15-210 inhibits the proliferation of cervical cancer SIHA cells by up-regulating the expression of CDKN1A gene.SNHG15-210 is a potential molecular target for cervical cancer.