献血者血小板表面ABO血型抗原表达强度分析

Expression of ABO blood group antigen on platelets of blood donors

目的研究献血者血小板表面ABO血型抗原表达水平。方法取正常ABO血型献血者506例,采用流式细胞术分析血小板表面ABO抗原。其中30例用于监测血小板保存期ABO抗原表达变化,476例用于献血人群血小板ABO抗原表达差异分析。结果在体外保存的1~7 d,血小板表面ABO抗原有轻微波动但相对稳定。根据平均荧光强度MFI可将献血人群分为低、中、高表达3群,平均频率分别为59.6%、35.5%和4.9%。AB型血小板的A和B抗原展示竞争性协同表达模式。结论大部分个体的血小板表面ABO抗原呈低表达,这些低表达个体可作为ABO非同型输注的潜在献血者,有助于改善血小板输注策略。

Objective To study the expression level of ABO blood group antigen on the surface of platelets of blood donors.Methods A total of 506 donors with normal ABO blood group were selected to analyze the ABO antigen on platelets by flow cytometry.Among them,30 donors were selected to monitor the changes of ABO antigen on platelets during the storage period,and the remaining 476 to analyze the expression difference of ABO antigen among blood donors.Results The ABO antigen on platelets of each sample fluctuated slightly but was relatively stable over 1~7 days in vitro.According to MFI values,the donors were divided into low-,moderate-and high-expression groups,with the average frequency of 59.6%,35.5%and 4.9%,respectively.The A and B antigens of blood group AB platelets exhibited a competitive co-expression pattern.Conclusion Most individuals have low-expression phenotype of ABO antigen on platelets and can be potential platelet donors for ABO-incompatibility transfusion,which is helpful to improve platelets transfusion strategy.