藤石松乙酸乙酯部位的化学成分研究

Chemical constituents of ethyl acetate extract from Lycopodiastrum casuarinoides

目的研究藤石松的化学成分。方法藤石松干燥全草用75%乙醇提取,所得流浸膏加3%酒石酸水溶液分散后,依次用石油醚、乙酸乙酯、正丁醇萃取,将所得的乙酸乙酯部分采用硅胶、ODS、聚酰胺及半制备HPLC等方法进行分离,根据化合物的理化性质和波谱数据进行结构鉴定。结果分离得到10个化合物,分别鉴定为呋喃甲醛(1)、富马酸单甲酯(2)、二氢阿魏酸甲酯(3)、二氢阿魏酸乙酯(4)、咖啡酸乙酯(5)、5,7-二羟基-2-甲基色原酮(6)、脱落酸(7)、苔色酸乙酯(8)、4-甲氧基苯甲酸(9)、香草酸(10)。结论化合物1~10均为首次从藤石松属植物中分离得到。

Objective To determine the chemical constituents from Lycopodiastrum casuarinoides.Methods The whole dry grass of Lycopodiastrum casuarinoides was extracted with 75% ethanol,and the liquid extract was suspended in 3% tartaric acid aqueous solution and extracted with petroleum ether,ethyl acetate and n-butanol,respectively.The ethyl acetate extract was isolated and purified by silica,ODS,polyamide and pre-HPLC.The structures of obtained compounds were identified by physicochemical properties and spectral data.Results Ten compounds were isolated and identified as furfural (1),monomethyl fumarate (2),dihydroferulic acid methyl ester (3),dihydroferulic acid ethyl ester (4),caffeic acid ethyl ester (5),5,7-dihydroxy-2-methyl chromone (6),abscisic acid (7),ethyl orsellinate (8),4-methoxybenzoic acid (9),and vanillic acid (10).Conclusion Compounds 1-10 have been isolated from the genus Lycopodiastrum for the first time.