6-姜烯酚对斑马鱼肝脏细胞炎症通路和抗氧化作用的影响

Effects of 6-shogaol on inflammation response and antioxidant capacity in zebrafish liver cells

为探究6-姜烯酚对鱼类的免疫调节作用,用不同浓度的6-姜烯酚孵育斑马鱼肝脏细胞48 h后,检测其生长率、胞内活性氧(ROS)水平、抗氧化酶活性,并进行抗炎、抗氧化基因表达量的检测。结果显示:高浓度的6-姜烯酚抑制肝脏细胞的增殖(P<0.05);6-姜烯酚抗氧化作用显著,20μmol/L处理组抗氧化酶SOD和GSH-PX的活性最高(P<0.05),而活性氧ROS和脂质过氧化产物MDA含量最低(P<0.05),相关基因表达分析表明其作用机制通过抑制keap1的表达来激活Keap1-Nrf2/HO-1-抗氧化酶信号通路;低浓度处理组6-姜烯酚上调了斑马鱼肝脏细胞NF-κB炎症通路细胞因子表达量(P<0.05),和对照组相比,抗炎因子表达量下降。上述结果表明,20μmol/L的6-姜烯酚能够促进斑马鱼肝脏细胞的抗氧化功能,不同浓度的6-姜烯酚均可以激活斑马鱼肝脏细胞的抗炎通路来启动非特异性免疫功能。

In order to explore the immune-regulation function of 6-shogaol in fish,the growth rate,the production of intracellular reactive oxygen(ROS)and lipid peroxidation(MDA),the antioxidant enzyme activity,and the expression of key genes in the inflammatory and antioxidant pathways were detected in zebrafish liver cells incubated with different concentrations of 6-shogaol for 48 h.The results showed that high concentrations of 6-shogaol inhibited the proliferation of liver cells(P<0.05).The antioxidant effect of 6-shogaol was significant,with the highest activity of antioxidant enzyme SOD and GSH-PX,and lowest production of ROS and MDA in the 20μmol/L treatment group(P<0.05).The gene expression analysis showed that the mechanism of action might be due to the activation of Keap1-Nrf2/HO-1-antioxidant enzyme signaling pathway by inhibiting the keap1 expression.6-shogaol increased the expression of inflammatory cytokine genes in the NF-κB inflammatory pathway(tnf-α,il-1β,il-6)in zebrafish liver cells(P<0.05).While in contrast to the control group,6-Shogaol down-regulated the expression of anti-inflammatory cytokine il-10.The above results showed that 20μmol/L 6-shogaol can promote the antioxidant function and 6-shogaol can activate the anti-inflammatory pathways of zebrafish liver cells to initiate immune response.