利用液质联用技术分析高温处理对人参皂苷的转化规律

Studies on the Ginsenosides Transform Regulation of Steam Ginseng with High Temperature by HPLC-ESI-MS

采用液质联用(HPLC-ESI-MS)技术研究了高温处理对人参皂苷的影响。对经过高温处理的人参与未经过处理的对照药材进行了系统研究,通过总离子流图与质谱图信息并结合人参皂苷结构分析了人参皂苷的裂解规律。结果表明,随着高温蒸制的进行,人参大分子量的皂苷发生水解,相应地生成了小分子量的皂苷。人参皂苷Re、Rg2生成了Rg6、F4,人参皂苷Rg1生成了Rh1、Rk3、Rh4,人参皂苷Rs1和Rs2生成了Rs3,人参皂苷Rb1、Rb2、Rb3、Rc、Rd等生成了人参皂苷20R-Rg3、20S-Rg3、Rk1、Rg5。人参经过高压蒸制,生成常压蒸制时所没有的皂苷。

HPLC-ESI-MS was used for the analyses of steamed ginseng with high temperature compare with the unprocessed ginseng,and the effect of high temperature treatment on Ginsenoside was studied.Using total ion chromatogram and tandem mass spectrometry combine with the formula of ginsenoside to obtain the regulation of transform of ginsenoside.The experimental results showed that the weight molecular ginsenoside hydrolyzed to the light molecular ginside when high pressure steam occurred.Re and Rg2 hydrolyze to Rh6 and F4,Rg1 hydrolyze to Rh1,Rk3 and Rh4,Rs1 hydrolyze to Rs3,Rb1,Rb2,Rb3,Rc and Rd hydrolyze to 20R-Rg3,20S-Rg3,Rk1,and Rg5.The results indicates that the ginseng is steamed under high pressure to produce saponins,which are absent in normal pressure steaming.