苦杏仁皮中膳食纤维和黄酮同步提取工艺的优化

Optimization of the simultaneous extraction technology of dietary fiber and flavonoids from the peel of Prunus armeniaca

目的优化苦杏仁皮中膳食纤维和黄酮的同步提取工艺。方法采用酶-重量法计算膳食纤维含量,采用紫外分光光度法测定黄酮含量;以料液比、pH、木瓜蛋白酶溶液浓度、α-淀粉酶溶液浓度、酶解温度和酶解时间为因素,膳食纤维和黄酮含量为指标,采用单因素实验和正交实验设计优化提取工艺。结果最优提取工艺为料液比1∶10(g/mL)、pH5、木瓜蛋白酶溶液浓度0.5%、α-淀粉酶溶液浓度0.5%、酶解温度50℃、酶解时间1 h。经3次实验验证,得膳食纤维的平均含量为0.801 g/g(RSD=1.95%),黄酮的平均含量为2.135 mg/g(RSD=2.44%),平均综合评分为0.988分(RSD=0.81%)。结论所得最优提取工艺稳定、可行。

OBJECTIVE To optimize the simultaneous extraction technology of dietary fiber(DF)and flavonoids from the peel of Prunus armeniaca.METHODS The content of DF was calculated with enzyme-gravimetric method,and the content of flavonoids was determined by ultraviolet spectrophotometry.The orthogonal design and single factor test were used to optimize the extraction technology,with the factors of liquid-solid ratio,pH,papain concentration,α-amylase concentration,temperature of enzymatic hydrolysis and time of enzymatic hydrolysis as factors,using the contents of DF and flavonoids as indexes.RESULTS The optimal extraction technology included the solid-liquid ratio of 1∶10(g/mL),pH5,0.5%papain and 0.5%α-amylase,enzymatic hydrolysis at 50℃for 1 h.After three times of validation,the average content of DF was 0.801 g/g(RSD=1.95%),and the average content of flavonoids was 2.135 mg/g(RSD=2.44%).The average comprehensive score was 0.988(RSD=0.81%).CONCLUSIONS The optimal extraction technology is stable and feasible.