奶牛乳房炎金黄色葡萄球菌的分离鉴定

Isolation and Identification of Staphylococcus Aureus from Cow Mastitis

[目的]为检测江苏省某奶牛场中乳房炎是否为金黄色葡萄球菌所致,采取4份患病乳汁样本进行分离鉴定,并针对金黄色葡萄球菌采用分离后鉴定。[方法]通过细菌分离鉴定、涂片染色镜检、生化实验这几种方法。[结果]分离到的2株致病菌为金黄色葡萄球菌,以及一株大肠杆菌和一株肠球菌。[结论]本次检测的奶牛场乳腺炎为多种致病病原菌同时引起,主要为金黄色葡萄球菌感染。

[Objective]In order to detect whether mastitis is caused by Staphylococcus aureus in a dairy farm in Heilongjiang province,four milk samples were taken for experimental isolation and identification,and Staphylococcus aureus was identified after isolation.[Methods]Bacterial isolation and identification,smear staining microscopic examination,biochemical experiments.[Results]Results shows that the 4 isolated strains were Escherichia coli,Enterococcus,etc.,and 2 strains of Staphylococcus aureus.[Conclusion]The conclusion shows that cow mastitis was caused by many kinds of bacteria,and the main pathogen was Staphylococcus aureus.