CRISPR/Cas技术在抗除草剂作物育种中的研究与应用进展

Research and Application Progress of CRISPR/Cas Technology in Herbicide‑resistant Crops Breeding

CRISPR/Cas系统是一种简单、低成本、高效、精准的基因编辑技术,该技术能够进行基因的定向改造,加速新品种培育进程,在种质资源创制中的应用潜力较高。概述了CRISPR/Cas系统的技术原理及其在作物抗除草剂育种中的应用,简要指出了目前CRISPR/Cas技术在抗除草剂种质创制及应用过程中存在的问题及发展方向,以期为今后利用CRISPR/Cas技术创制抗除草剂新种质提供理论依据。

CRISPR/Cas system is a simple,inexpensive,high efficiency,and precise tool for genome editing.It can precise genome modification and speed up breeding process,which has great potential in creating novel germplasm.This paper summa‑rized CRISPR/Cas toolkits as well as their application in herbicide-resistant crops breeding,and briefly introduced the current challenges and future application of CRISPR/Cas technology for developing herbicide-resistant crops.The paper aimed to provide a theoretical basis for generating herbicide-resistant crops with CRISPR/Cas system in the future.