萝卜ALMT基因家族的鉴定与表达

Genome-wide identification and expression analysis of ALMT gene family in radish

为探究萝卜铝激活苹果酸转运蛋白(ALMT)家族成员对生物和非生物胁迫的响应,本研究通过生物信息学方法对萝卜ALMT家族成员进行鉴定,并利用转录组数据对其进行表达分析。结果显示,萝卜基因组中包含17个ALMT基因,分布于8条染色体。该家族成员外显子数量为5~7个,预测N-端含有5~6个跨膜结构,在染色体上的分布不均匀。萝卜ALMT基因家族成员启动子上有多种激素响应、胁迫响应、组织和器官生长发育与环境响应的顺式作用元件,表明萝卜ALMT基因家族成员的表达受多种条件的调控。对它们在不同组织和发育时期,以及对Agrobacterium tumefaciens的侵染和重金属(铅、镉和铬)胁迫的响应研究发现,RsALMT1和RsALMT13可能参与抵抗A.tumefaciens的侵染;RsALMT3可能调控叶片气孔的开放,而RsALMT7、RsALMT11和RsALMT14可能调控叶片气孔的关闭;RsALMT15可能参与绿肉萝卜根肉中叶绿素的积累;RsALMT16在抵御铅胁迫过程中发挥重要作用。以上结果将为进一步研究萝卜ALMT家族成员在生物和非生物胁迫响应中的功能与分子机制奠定基础。

In order to explore the response of aluminium activated malate transporter(ALMT)genes to biotic and abiotic stress in radish,ALMT genes of radish were genome-wide identified by bioinformatics method and expression patterns were analyzed based on RNA-seq data.The results showed that there were 17 ALMT genes in radish genome,and distributed on eight chromosomes.Exon number of these genes ranged from 5 to 7,and 5-6 transmembrane domains were predicted in their N-terminal region.Number of RsALMT genes on chromosomes was different.Expression analysis of RsALMT genes in different tissues and developments stages,treatment with Agrobacterium tumefaciens and heavy metal(cadmium,chromium and lead)showed that RsALMT1 and RsALMT13 might be involved in resistance to the infection of A.tumefaciens,RsALMT3 might control stomatal open,while RsALMT7,RsALMT11 and RsALMT14 mightbe involved in stomatal closure.RsALMT15 might regulate the accumulation of chlorophyll in flesh of radish and RsALMT16 might be involved in resistance to leadstress.Therefore,the above results would provide a theoretical basis for further study on the functions and molecular mechanisms of radish ALMT family members in response to biotic and abiotic stress.