超声辅助低共熔溶剂法提取玉米芯总黄酮工艺优化研究

Optimization of Ultrasonic-Assisted Deep Eutectic Solvent Extraction of Total Flavonoids from Corncob

为利用超声法辅助低共熔溶剂提取玉米芯中的总黄酮,首先通过筛选实验,确定提取溶剂组成和组分比例;再以提取温度、液料比、超声功率及提取时间为变量,总黄酮提取量为响应值,采用响应面分析法优化提取工艺。结果显示,以含水量为30%的氯化胆碱/乙二醇(摩尔比1:3)作为溶剂提取玉米芯总黄酮的最优工艺为:在20:1 mL/g的液料比条件下,选择功率137 W、61℃、超声提取19.5 min。此时,玉米芯总黄酮的实际提取量为4.180 mg/g,与模型预测值误差为0.71%,证明了该工艺稳定、可靠,可为玉米芯中黄酮类化合物的开发利用提供科学数据。

Total flavonoids from corncob were extracted using ultrasonic-assisted deep eutectic solvent. Firstly, the extraction solvent composition and component ratio were determined by screening experiment. Then response surface methodology was used to optimize the extraction process. The extraction temperature, liquid-solid ratio, ultrasonic power and extraction time were used as variables, and the extraction amount of total flavonoids was used as response value. The results showed that the optimum extraction process of total flavonoids from corncob with choline chloride/ethylene glycol(molar ratio 1:3) with water content of 30% was as follows: Temperature 61 ℃, 20:1(mL/g) of liquid-solid ratio,ultrasonic power 137 W, and extraction time 19.5 min, the extraction amount of flavonoids was 4.180 mg/g, with an error of0.71% compared with the predicted value. The method was proved to be stable and reliable, and could provide scientific data for the development and utilization of flavonoids in corncob.